The Roles of Calcium Pulses, Waves, and Gradients in Early Development

钙脉冲、波和梯度在早期发育中的作用

• 批准号: 8811198
• 负责人: Lionel Jaffe
• 金额: $ 22.86万
• 依托单位: Marine Biological Laboratory
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-11-01 至 1992-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8811198&HistoricalAwards=false
• 关键词: Roles; Calcium; Pulses; Waves; Gradients

The roles of free cytosolic calcium pulses, waves, and gradients in the early development of ascidian eggs as well as of cellular slime molds will be explored. The proposal is particularly concerned with the roles of calcium in establishing developmental pattern in these systems, i.e., in ooplasmic segregation in the Ciona or Phallusia egg; and in establishing a pattern of prestalk and spore cells in Dictyostelium. These explorations will be carried out in three main ways: 1) Directly visualize free calcium changes and patterns with the aid of the photoprotein aequorin and an imaging photon detector. 2) Specifically perturb (and thus study the roles) of these calcium patterns with various means which will include the injection of newly synthesized weak calcium buffers. 3) Examine the transcellular calcium currents which help produce these free calcium patterns by using a newly developed calcium-specific vibrating extracellular electrode. %%% The development of an early embryo is controlled by gradients of informational molecules localized in different regions of the unfertilized and newly fertilized egg. One of the major components involved in establishing this information appears to be coupled to calcium distribution itself. Dr. Jaffe will test this idea directly using reagents and detectors originally developed in his laboratory.

将探讨游离胞质钙脉冲、波和梯度在海鞘卵和细胞粘菌早期发育中的作用。 该提案特别关注钙在这些系统中建立发育模式中的作用，即在玻璃海鞘或阴茎体卵的卵质分离中的作用；以及建立盘基网柄菌属前柄和孢子细胞的模式。 这些探索将通过三种主要方式进行：1）借助光蛋白水母发光蛋白和成像光子探测器直接可视化游离钙的变化和模式。 2）用各种方法特别扰乱（并因此研究其作用）这些钙模式，其中包括注射新合成的弱钙缓冲液。 3) 使用新开发的钙特异性振动细胞外电极检查跨细胞钙电流，这有助于产生这些游离钙模式。 %%% 早期胚胎的发育是由位于未受精卵和新受精卵不同区域的信息分子梯度控制的。 建立此信息所涉及的主要组成部分之一似乎与钙分布本身有关。 贾菲博士将使用最初在他的实验室开发的试剂和检测器直接测试这个想法。