The Roles of Calcium Pulses, Waves, and Gradients in Early Development

游离胞浆钙脉冲、波和梯度的作用 在海鞘卵和细胞的早期发育中， 将探索黏菌。 该提案特别是 关注钙在建立发育过程中的作用 这些系统中的模式，即，在卵质分离中， 玻璃海鞘或Phallusia卵;并在建立一个模式的prestalk 和孢子细胞。 这些探索将是 主要通过三种方式进行：1）直接可视化自由 钙的变化和模式与援助的光蛋白 水母发光蛋白和成像光子检测器。 2)特别是扰动 (and从而研究这些钙模式的作用）， 这将包括注入新合成的弱 钙缓冲剂。 3)检查跨细胞钙电流 它通过使用一种新的 研制了钙离子特异性振动细胞外电极。 %%% 早期胚胎的发育是由 信息分子定位在不同的区域， 未受精和刚受精的卵。 的一个主要 参与建立此信息的组件似乎 与钙的分布有关。 杰斐医生会测试 这种直接使用试剂和检测器的想法 在他的实验室里开发的。

The roles of free cytosolic calcium pulses, waves, and gradients in the early development of ascidian eggs as well as of cellular slime molds will be explored. The proposal is particularly concerned with the roles of calcium in establishing developmental pattern in these systems, i.e., in ooplasmic segregation in the Ciona or Phallusia egg; and in establishing a pattern of prestalk and spore cells in Dictyostelium. These explorations will be carried out in three main ways: 1) Directly visualize free calcium changes and patterns with the aid of the photoprotein aequorin and an imaging photon detector. 2) Specifically perturb (and thus study the roles) of these calcium patterns with various means which will include the injection of newly synthesized weak calcium buffers. 3) Examine the transcellular calcium currents which help produce these free calcium patterns by using a newly developed calcium-specific vibrating extracellular electrode. %%% The development of an early embryo is controlled by gradients of informational molecules localized in different regions of the unfertilized and newly fertilized egg. One of the major components involved in establishing this information appears to be coupled to calcium distribution itself. Dr. Jaffe will test this idea directly using reagents and detectors originally developed in his laboratory.