Roles of Calcium Pulses, Waves and Gradients in Early Development

Jaffe博士将继续探索游离胞质中 钙脉冲，波和梯度的发展， 胚胎模式，在胞质分裂以及 受精他的主要目的是发现和描述 钙离子的时空分布模式， 进化，并探索其发展作用， 机制等他将这些图案可视化的主要方法 注射荧光钙特异性蛋白质 水母发光蛋白。他还将使用振动钙特异性电极 探索细胞表面钙电流的模式。他 将继续在岩藻、海鞘、非洲爪蟾和 青鳉蛋以及其他一些系统。的一个主要 修改的方法，从而探索的作用，这些 钙模式将抑制他们通过注射 适当的钙缓冲剂。杰斐博士也会修改 通过适当应用钙通道阻滞剂的模式 和激动剂以及钙离子载体。该企业 应该证明对理解细胞具有广泛价值 因为钙离子是一种相对较小的 一类小物质（如氢离子，ATP，环AMP， IP3等）其细胞内模式是 细胞内的生理控制机制。

Dr. Jaffe will continue to explore the roles of free cytosolic calcium pulses, waves and gradients in the development of embryological pattern, in cytokinesis as well as in fertilization. His broad purpose is to discover and describe spatiotemporal calcium patterns which have been conserved in evolution and to explore their developmental roles and mechanisms. His main method for visualizing these patterns will be to inject chemiluminescent, calcium specific proteins called aequorins. He will also use vibrating calcium specific electrodes to explore patterns of calcium currents through cell surfaces. He will continue this exploration in fucoid, ascidian, Xenopus and medaka eggs as well as in some other systems. One of the main methods for modifying, and thus for exploring the role of, these calcium patterns will be to suppress them by injecting appropriate calcium buffers. Dr. Jaffe will also modify such patterns via appropriate applications of calcium channel blockers and agonists as well as calcium ionophores. This enterprise should prove to be of broad value in understanding cell development because calcium ions are among a relatively small class of small substances (like hydrogen ions, ATP, cyclic AMP, IP3, etc.) whose intracellular patterns are central to the physiological control mechanisms within cells.