Roles of Calcium Pulses, Waves and Gradients in Early Development

钙脉冲、波和梯度在早期发育中的作用

• 批准号: 9103569
• 负责人: Lionel Jaffe
• 金额: $ 27万
• 依托单位: Marine Biological Laboratory
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1995-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9103569&HistoricalAwards=false
• 关键词: Roles; Calcium; Pulses; Waves; Gradients

Dr. Jaffe will continue to explore the roles of free cytosolic calcium pulses, waves and gradients in the development of embryological pattern, in cytokinesis as well as in fertilization. His broad purpose is to discover and describe spatiotemporal calcium patterns which have been conserved in evolution and to explore their developmental roles and mechanisms. His main method for visualizing these patterns will be to inject chemiluminescent, calcium specific proteins called aequorins. He will also use vibrating calcium specific electrodes to explore patterns of calcium currents through cell surfaces. He will continue this exploration in fucoid, ascidian, Xenopus and medaka eggs as well as in some other systems. One of the main methods for modifying, and thus for exploring the role of, these calcium patterns will be to suppress them by injecting appropriate calcium buffers. Dr. Jaffe will also modify such patterns via appropriate applications of calcium channel blockers and agonists as well as calcium ionophores. This enterprise should prove to be of broad value in understanding cell development because calcium ions are among a relatively small class of small substances (like hydrogen ions, ATP, cyclic AMP, IP3, etc.) whose intracellular patterns are central to the physiological control mechanisms within cells.

Jaffe博士将继续探索游离胞质钙脉冲、波和梯度在胚胎学模式发育、细胞质分裂和受精中的作用。他的主要目的是发现和描述在进化中保存的时空钙模式，并探索其发育作用和机制。他将这些模式可视化的主要方法是注入化学发光的钙特异性蛋白质，称为aequorins。他还将使用振动钙特异电极来探索钙电流通过细胞表面的模式。他将继续在岩藻类、海鞘类、爪蟾类和medaka类以及其他一些系统中进行探索。其中一个主要的方法来修改，从而探索这些钙模式的作用，将是通过注射适当的钙缓冲液来抑制它们。Jaffe博士还将通过适当应用钙通道阻滞剂和激动剂以及钙离子载体来修改这种模式。这项研究将被证明对理解细胞发育具有广泛的价值，因为钙离子是一类相对较小的小物质（如氢离子、ATP、环AMP、IP3等），它们的细胞内模式是细胞内生理控制机制的核心。