Signal Transduction Across Plant Plasma Membranes

跨植物质膜的信号转导

• 批准号: 8811465
• 负责人: Philip Low
• 金额: $ 15万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1991-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8811465&HistoricalAwards=false
• 关键词: Signal; Transduction; Across; Plant; Plasma

Plant cells synthesize a variety of defense compounds upon stimulation with pathogen-derived or plant cell wall-derived molecules termed elicitors. While much is known concerning the nature of these elicitors and the types of defense molecules produced in response to elicitation, virtually nothing is known about the sequence of events involved in transducing the extracellular elicitor signal into the cell's nucleus. The objective of this research is to identify those signal transduction and amplification processes which occur at the plasma membrane during elicitation. Using signal transduction pathways in animal cells as models, the goals of this project are to evaluate the possible participation of the following signaling mechanisms during elicitation of phytoalexin formation: 1) calcium transport, 2) proton transport, 3) changes in membrane potential, 4) changes in trans-plasma membrane dehydrogenase activity, and 5) changes in phosphatidylinositol turnover. Emphasis will be placed on mechanisms 1, 2, and 3. A detailed understanding of signal transduction processes in plant cells is of great intrinsic interest and may eventually contribute to the rational design of strategies for manipulating the various defense and growth responses in plants.

植物细胞在病原体来源或植物细胞壁来源的分子（称为激发子）刺激下合成多种防御化合物。 虽然人们对这些引发子的性质和响应引发而产生的防御分子的类型了解很多，但实际上对将细胞外引发子信号转导到细胞核中所涉及的事件顺序一无所知。 本研究的目的是确定诱导期间质膜上发生的信号转导和放大过程。 使用动物细胞中的信号转导途径作为模型，该项目的目标是评估以下信号传导机制在引发植物抗毒素形成过程中可能的参与：1）钙转运，2）质子转运，3）膜电位的变化，4）跨质膜脱氢酶活性的变化，以及5）磷脂酰肌醇周转的变化。 重点将放在机制 1、2 和 3 上。详细了解植物细胞中的信号转导过程具有重大的内在意义，并且最终可能有助于合理设计操纵植物中各种防御和生长反应的策略。