Signal Transduction Across Plant Plasma Membranes

跨植物质膜的信号转导

• 批准号: 9303929
• 负责人: Philip Low
• 金额: $ 47.87万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-01-15 至 1998-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9303929&HistoricalAwards=false
• 关键词: Signal; Transduction; Across; Plant; Plasma

9303929 Low One of the most rapid defense responses of a plant to an invading pathogen is the oxidative burst, where, within 5 minutes of the encounter of the plant cell with a pathogen-derived elicitor molecule there is the sudden release of hydrogen peroxide and other oxidative species. The oxidative burst is possibly the plant's first line of defense against infection, and appears to result from one of the simplest and most topologically localized signal transduction pathways. The overall goal of this research is to identify and characterize the second messengers and signaling intermediates that communicate the association of an elicitor molecule with its cell surface receptor to the oxidase complex that produces oxygen radicals and hydrogen peroxide. Intermediate goals are to: 1) identify purified elicitors that activate the oxidative burst pathway without stimulating other signaling pathways that lead to delocalized defense responses (e.g. production of phytoalexins or PR proteins), 2) identify second messengers that are activated by such elicitors, 3) rank the second messengers chronologically in order of their appearance, and 4) show linkage or connectivity between adjacent pairs of identified second messengers using messenger-specific inhibitors, activators, etc. %%% One of the most rapid defense responses of a plant cell to an invading pathogen is the "oxidative burst," the sudden release of hydrogen peroxide and other oxidative chemical species. The oxidative burst probably represents the first line of a plant's defense against infection. The goal of this research is to work out the chain of biochemical events between the detection of a pathogen, as signaled by the arrival of a pathogen-specific chemical on a specific plant cell receptor, through the resulting signal transduction pathways, to the production of oxidative chemicals. The results of this research will contribute substantially to our understanding of this important cellular signa ling pathway in plants and should provide considerable information on where and how the defense responses of plants can be genetically manipulated. ***

植物对入侵的病原体最快速的防御反应之一是氧化爆发，在植物细胞与病原体衍生的激发子分子相遇的5分钟内，会突然释放过氧化氢和其他氧化物质。氧化爆发可能是植物抵御感染的第一道防线，似乎是由最简单和最拓扑定位的信号转导途径之一引起的。本研究的总体目标是鉴定和表征第二信使和信号中间体，这些信使和信号中间体将激发子分子及其细胞表面受体与产生氧自由基和过氧化氢的氧化酶复合物联系起来。中期目标是：1)鉴定纯化的激发子，这些激发子激活氧化爆发途径，而不刺激导致脱域防御反应的其他信号通路（例如，植物抗毒素或PR蛋白的产生），2)鉴定被这些激发子激活的第二信使，3)根据其出现的时间顺序对第二信使进行排序，4)使用信使特异性抑制剂显示鉴定的第二信使相邻对之间的连锁或连接。植物细胞对入侵的病原体最迅速的防御反应之一是“氧化爆发”，即过氧化氢和其他氧化化学物质的突然释放。氧化爆发可能代表了植物抵御感染的第一道防线。本研究的目标是找出从病原体检测到病原体特异性化学物质到达特定植物细胞受体，通过由此产生的信号转导途径到氧化化学物质产生之间的生化事件链。这项研究的结果将大大有助于我们了解植物中这一重要的细胞信号通路，并为植物的防御反应在哪里以及如何被基因操纵提供重要的信息。＊＊＊