U.S.-Korea Cooperative Research on Lipid Second Messengers in Plant Signal Transduction

9600183 Low该奖项提供资金，允许普渡大学化学系Philip S.Low博士与韩国浦项科技大学生命科学系Young sook Lee博士进行为期24个月的关于植物信号转导中脂质第二信使的合作研究计划。合作者建议通过以下方法进一步了解脂源性第二信使在植物防御中的作用：(1)测试添加磷脂酶A或C反应产物是否可以独立地刺激培养细胞和完整植物中的氧化爆发；(2)确定氧化爆发的激发子是否也激活完整大豆和番茄中的磷脂酶A和C；以及(3)评估在寡聚半乳糖酸钠诱导的氧化爆发中存在缺陷的突变番茄植株在激活这些磷脂酶方面是否也存在缺陷。植物防御系统在暴露于病原体或来自病原体的特定分子时被激活，称为诱导子。到目前为止发现的最快速的植物防御反应之一是氧化爆发，在这个过程中，过氧化氢或过氧化氢离子在激发子与植物细胞表面受体结合后几分钟内释放。该项目将进行的实验有望确定磷脂酶A和C是否参与悬浮培养细胞以及完整的大豆和番茄植株中氧化爆发的信号传递。合作者在该项目领域具有丰富的研究经验。然而，刘特佐博士的经验主要是培养细胞分析，而李博士则专注于完整的植物系统。他们的互补专业知识将是这项植物防御系统研究的宝贵财富。该项目与美韩合作科学计划的目标相关，该计划旨在通过交流科学信息、思想、技能和技术以及通过在互利问题上的合作来提高美韩科学家和S工程师之间的合作水平。韩国参与该项目得到了韩国科学与工程基金会(KOSEF)的支持。该项目增加了国际合作的层面，国际和平研究所的研究在美国国家科学基金会补助金编号。MCB-9303929。***

9600183 Low This award provides funds to permit Dr. Philip S. Low, Department of Chemistry, Purdue University, to pursue with Dr. Youngsook Lee, Department of Life Sciences, Pohang University of Science and Technology, Korea, for 24 months, a program of cooperative research on lipid second messengers in plant signal transduction. The collaborators propose to further their understanding of the roles of lipid-derived second messengers in plant defense by (1) testing whether addition of phospholipase A or C reaction products can independently stimulate the oxidative burst in both cultured cells and in intact plants, (2) determining whether elicitors of the oxidative burst also activate phospholipases A and C in intact soybean and tomato plants, and (3) evaluating whether mutant tomato plants that are defective in the oligogalacturonide-induced oxidative burst are also defective in activating these phospholipases. Plant defense systems are activated upon exposure to pathogens or to specific molecules derived from pathogens, termed elicitors. One of the most rapid plant defense responses identified to date is the oxidative burst, a process whereby hydrogen peroxide or peroxide ions are released within minutes of elicitor binding to plant cell surface receptors. The experiments to be undertaken in this project are expected to establish whether phospholipases A and C participate in signaling the oxidative burst in both suspension cultured cells and in intact soybean and tomato plants. The collaborators are experienced in research in the field of this project. Dr. Low's experience, however, is primarily with cultured cell assays, while Dr. Lee has concentrated on intact plant systems. Their complementary expertise will be a valuable asset in this study of plant defense systems. This project is relevant to the objectives of the U.S.-Korea Cooperative Science Program which seeks to increase the level of cooperation between U.S. and Korean scientists and engineer s through the exchange of scientific information, ideas, skills, and techniques and through collaboration on problems of mutual benefit. Korean participation in the project is supported by the Korea Science and Engineering Foundation (KOSEF). This project adds an international cooperative dimension to the PI's research under NSF Grant No. MCB-9303929. ***