US-Sweden Cooperative Research: Investigation of InterfacialPhenomena by Ellipsometry, Acoustic Shear, and Electro- chemical Techniques

美国-瑞典合作研究：通过椭圆偏振、声剪切和电化学技术研究界面现象

• 批准号: 8813562
• 负责人: David Paul
• 金额: $ 1.09万
• 依托单位: University of Arkansas
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-02-01 至 1993-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8813562&HistoricalAwards=false
• 关键词: US; Sweden; Cooperative; Research; Investigation

This award will support collaborative research between Dr. David Paul, University of Arkansas, and Professor Ingemar Lundstrom, Department of Physics and Measurement Technology, Linkoping University, Sweden. The objectives of this research are to clarify how the piezoelectric crystal (quartz crystal microbalance) functions as a sensor in the liquid phase. In addition, the investigators will study how the microviscosity of the sensor/solution interface is affected by conformation changes in large protein molecules next to the surface. If the potentially useful piezoelectric sensor is to be used to analyze important biological molecules, fundamental questions regarding protein interactions with the surface of the sensor must be answered. It is proposed that the interaction of "simple" proteins with the mechanically oscillating surface of the sensor can be completely described with the data collected from combined ellipsometric and electrochemical experiments. This joint effort will bring together Swedish expertise in ellipsometry with U.S. expertise in piezoelectric sensors to examine this question. The results of this research should provide new insights into the use of piezoelectric crystals for sensors and add to the information on how solvents and proteins interact with metal surfaces. Discovering which variables in the sensor surface/solution interface cause a frequency shift should be beneficial in applying the sensor to practical systems. In addition, measurements from the piezoelectric sensor can also provide information on how the microviscosity of the liquid in contact with the protein is affected by conformational changes of the protein. This data may then be used to further develop practical applications of the piezoelectric sensor in the liquid phase. Potential applications are in the areas of biotechnology, clinical analysis, and genetic engineering.

该奖项将支持大卫博士与 阿肯色州大学的保罗和英格玛·伦德斯特罗姆教授， 林雪平物理与测量技术系 瑞典大学。 本研究的目的是澄清 压电晶体（石英晶体微量天平） 在液相中用作传感器。 此外该 研究人员将研究如何微粘度的 传感器/溶液界面受构象变化的影响， 大的蛋白质分子靠近表面。 如果潜在的 有用的压电传感器是用来分析重要的 生物分子，关于蛋白质的基本问题 必须响应与传感器表面的相互作用。 它 提出了“简单”蛋白质与 传感器的机械振荡表面可以完全 描述的数据收集的组合椭偏和 电化学实验 这一共同努力将使 瑞典的椭圆偏振技术和美国的 压电传感器来研究这个问题。 这项研究的结果应该提供新的见解， 使用压电晶体的传感器和添加到 关于溶剂和蛋白质如何与金属相互作用的信息 表面。 发现传感器中的哪些变量 引起频移表面/溶液界面应当 有利于将传感器应用于实际系统。 在 此外，来自压电传感器的测量还可以 提供关于液体的微粘度如何在 与蛋白质的接触受到构象变化的影响， 蛋白质。 这些数据可以用于进一步开发 压电传感器在液体中的实际应用 相位 潜在的应用领域是生物技术， 临床分析和基因工程。