US-Sweden Cooperative Research: Biochemical Studies of Pheromone Perception in Agrotis Segetum

美国-瑞典合作研究：小地老虎信息素感知的生化研究

• 批准号: 9014102
• 负责人: Glenn Prestwich
• 金额: $ 1.7万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-15 至 1994-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9014102&HistoricalAwards=false
• 关键词: US; Sweden; Cooperative; Research; Biochemical

This award will support collaborative research between Dr. Glenn Prestwich, State University of New York at Stony Brook and two Swedish researchers from the University of Lund, Sweden: Professor Christer Lofstedt of the Ecological Center and Professor Tommy Liljefors of the Chemical Center. The objective of the proposed project is to investigate mechanisms of pheromone detection. Chemists and biologists have developed considerable sophisti- cation in the elucidation of pheromone structures, industrial- scale pheromone synthesis, and in the use of pheromones for mating disruption and integrated pest management, since the publication of the first pheromone structure over thirty years ago. In contrast, current understanding of how pheromones work on a molecular level is still very rudimentary. Dr. Prestwich and his Swedish colleagues have worked together over the past few years on the chemistry, biochemistry, ecology, behavior and sensory physiology of pheromone perception in the turnip moth Agrotis segetum. Using pheromones prepared at Stony Brook, the investigators now propose to conduct: 1) biochemical studies of pheromone receptor and binding proteins, 2) computer simulations and electrophysiological evaluations of pheromone analogs, and 3) behavioral testing of physiologically active analogs. Dr. Prestwich will use molecular graphics programs at the University of Lund to evaluate conformations of pheromone analogs synthesized at Stony Brook, and will also use the routinely available supply of Agrotis segetum pupae and adult moths at Lund for biochemical experiments requiring live, staged insects. In addition, he will establish as routine a variety of biochemical techniques in the Lund laboratories and provide new structures for calculations and model testing by the Swedish groups. This research will benefit from the complementary expertise of Dr. Prestwich and his Swedish collaborators in the chemistry of pheromone structure. The results of this research will have important applications for future uses of pheromone technology, such as pest management.

该奖项将支持博士之间的合作研究。 Glenn Prestwich，斯托尼布鲁克纽约州立大学 和两名来自隆德大学的瑞典研究人员， 瑞典：生态学教授克里斯特·洛夫斯泰特 中心和教授汤米Liljefors的化学中心。 拟议项目的目的是调查 信息素检测机制。 化学家和生物学家已经发展了相当多的诡辩- 阳离子在信息素结构的阐明，工业- 规模信息素合成，并在使用信息素， 交配中断和综合虫害管理，因为 出版的第一个信息素结构超过30 年前 与此相反，目前对如何 信息素在分子水平上的作用还很初级。 普雷斯特维奇博士和他的瑞典同事们 在过去的几年里，化学，生物化学， 信息素的生态、行为和感觉生理 萝卜蛾Agrotis segetum的感知。 使用 信息素是在斯托尼布鲁克准备的，调查人员现在 拟开展：1）信息素的生物化学研究 受体和结合蛋白，2）计算机模拟和 信息素类似物的电生理学评价，和3） 生理活性类似物的行为测试。 Prestwich博士将使用分子图形程序， 隆德大学评估信息素的构象 在斯托尼布鲁克合成的类似物，也将使用 常规供应的小地老虎和成虫 在隆德进行的生物化学实验需要活的， 舞台昆虫此外，他还将建立一个 隆德实验室的各种生化技术 并为计算和模型试验提供新的结构 瑞典团体。 这项研究将受益于 Prestwich博士和他的瑞典人的互补专业知识 信息素结构化学方面的合作者。 的 这项研究的结果将有重要的应用， 信息素技术的未来用途，如害虫管理。