Analytical Studies of Binding Protein Interactions with Enzyme-Ligand Conjugates

该项目属于分析和曲面的常规领域 化学和生物分析化学的子领域。的 计划进行的研究集中在三个相关的研究领域。 这三个方面包括：1）开发分析程序 对于选定的蛋白质和除草剂， 方法学，2）使用中空透析纤维来开发一种 连续和可逆的生物分子检测方案，3） 选择单克隆抗体的基础实验 具有所需结合特性的抗配体抗体 但很少或没有桥连基团识别。 这里计划的研究旨在开发基于 某些蛋白质的选择性相互作用。这些测定 应该几乎一样有选择性，但要比 基于使用抗体的测定。此外，新 该方法不应要求使用放射性标记。

This project is in the general area of Analytical and Surface Chemistry and in the subfield of bioanalytical chemistry. The studies planned here focus on three related areas of research. These three areas include 1) developing analytical procedures for selected proteins and herbicides using simple homogeneous methodology, 2) the use of hollow dialysis fibers to develop a continuous and reversible biomolecule detection scheme, 3) fundamental experiments designed to select monoclonal anti-ligand antibodies with desirable binding characteristics but little or no bridging group recognition. The studies planned here are designed to develop assays based on the selective interactions of certain proteins. These assays should be almost as selective, but much less expensive than assays based on the use of antibodies. In addition, this new methodology should not require the use of radioactive labels.