New Directions for Enzyme-Linked Binding Assays

该项目属于分析和表面化学的一般领域，以及生物分析的子领域。在这项为期三年的持续资助期间，迈耶霍夫教授和他的学生将继续进行与在几种生物分子分析的新结合分析方法中使用酶作为标记有关的基础研究。利用酶联结合分析(ELBAS)将进行三个具体的研究，即(1)开发和研究一种新的基于凝集素的均相ELBA，其目的是合成更有用的酶-寡糖结合物，并证明这种偶联/凝集素系统可用于快速探测完整糖蛋白的碳水化合物结构；(2)评估使用多个酶标记和多个粘合剂同时进行单一混合物中生物分子的ELBA的适用性和分析限度；以及(3)探索实现底物的原位电化学释放的可行性，以潜在地用于ELBAS中特定蛋白质的检测。%这项研究建立在美国国家科学基金会拨款的基础上-

This project is in the general area of analytical and surface chemistry and in the subfield of bioanalysis. During the tenure of this three-year continuing grant, Professor Meyerhoff and his students will pursue fundamental studies relating to the use of enzymes as labels in several novel binding assay methods of analysis of biomolecules. Three specific research thrusts utilizing enzyme-linked binding assays (ELBAs) will be pursued, namely (1) the development and study of a new lectin-based homogeneous ELBA, with the specific intent of synthesizing more useful enzyme-oligosaccharide conjugates and demonstrating that such conjugate/lectin systems can be used to probe rapidly the carbohydrate structures of intact glycoproteins; (2) assessment of the applicability and analytical limits of using multiple enzyme labels in conjunction with multiple binders to perform simultaneous ELBAs of biolmolecules in a single mixture; and (3) exploration of the feasibility of implementing in situ electrochemical release of substrates for potential use in ELBAs for the detection of specific proteins. %%% This research builds upon results obtained under NSF grant CHE-