REU: Mammalian Systematics and Mitochondrial Gene Sequences

哺乳动物线粒体基因的模式和过程 将研究核苷酸序列水平的进化。 这项研究的主要重点将是在率 细胞色素c氧化酶亚基II（COII）的序列变化 基因编码的基因。 这个特殊的基因 直接与细胞色素c基因相互作用。 COII 基因的氨基酸取代量不同， 发生在特定的哺乳动物群体中，这一观察结果是 在啮齿类动物和灵长类动物的比较中非常明显。 的 本研究的主要策略是扩增DNA片段 使用聚合酶链反应， (PCR)以及扩增的单链DNA的测序。 这 将使用一个程序来评估COII的模式和克里思 啮齿类、灵长类和相关哺乳动物目的基因变化。 这些数据将提供直接信息， 这种基因的差异变化率， 由核和线粒体基因组编码的基因，以及 特定哺乳动物之间和内部的进化关系 组

The patterns and processes of mammalian mitochondrial gene evolution at the nucleotide sequence level will be investigated. The primary focus of this research will be on the rate of sequence change in the cytochrome c oxidase subunit II (COII) gene encoded in the mitochondrion. This particular gene interacts directly with the nuclear cytochrome c gene. The COII gene varies in the amount of amino acid substitutions that have occurred in particular mammalian groups, and this observation is pronounced in comparisons of rodents versus primates. The primary strategy of this research is to amplify DNA fragments containing the COII gene using the polymerase chain reaction (PCR) and the sequencing of amplified single stranded DNA. This procedure will be used to evaluate the mode and tempo of COII gene change in rodents, primates, and related mammalian orders. These data will provide direct information on the extent of differential rates of change in this gene, the coevolution of genes encoded by the nuclear and mitochondrial genomes, and the evolutionary relationships among and within particular mammalian groups.