Regulation of Plant Enzymes

植物酶的调节

• 批准号: 8815980
• 负责人: Bob Buchanan
• 金额: $ 45.28万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-02-15 至 1993-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8815980&HistoricalAwards=false
• 关键词: Regulation; Plant; Enzymes

This is a 4 year research program to elucidate the mechanism of regulation of enzymes controlling photosynthesis. One pivotal mechanism is the cytosolic fructose-2,6-bisphosphate system that regulates sucrose synthesis and breakdown in leaves. The objectives are to: (1) purify and characterize at the protein and gene levels newly identified leaf enzymes that synthesize and degrade fructose-2,6-bisphosphate; (2) determine whether these same activities of nonphotosynthetic (sink) tissues containing amyloplasts reside on enzymes similar to those of leaves. In parallel, studies will be continued on the chloroplast ferredoxin-thioredoxin system, a protein-mediated mechanism that links light to the regulation of enzymes functional in photosynthesis and related processes. These studies will (1) deduce the importance of catalytically active thiols in the photoregulation of target enzymes in isolated chloroplasts and leaves, and (2) characterize the remaining poorly understood component of this system--the novel enzyme ferredoxin-thioredoxin reductase (FTR)--with respect to mechanism of action, crystal structure, and EPR/redox properties. This research will increase our understanding of how plants synthesize and store starch and sucrose, the major reservoirs of edible carbohydrate.***

这是一个为期4年的研究计划，以阐明机制 控制光合作用的酶的调节。 一 关键机制是胞浆果糖-2，6-二磷酸 调节蔗糖合成和分解的系统， 叶子 目的是：（1）纯化和表征 在蛋白质和基因水平上， 合成和降解果糖-2，6-二磷酸;（2） 确定这些非光合作用的活动 含有造粉体的（汇）组织存在于酶上 类似于叶子的叶子。 与此同时，研究将 继续对叶绿体铁氧还蛋白-硫氧还蛋白系统， 蛋白质介导的机制，将光与调节 在光合作用中起作用的酶以及 流程. 这些研究将（1）推导出 催化活性硫醇在光调节靶 分离的叶绿体和叶中的酶，和（2） 描述剩余的知之甚少的成分， 这个系统--铁氧还蛋白-硫氧还蛋白 还原酶（FTR）-关于作用机制，晶体 结构和EPR/氧化还原性质。 这项研究将增加我们对植物 合成和储存淀粉和蔗糖， 可食用的碳水化合物 *