Gene regulation of active oxygen scavenging enzymes and breeding for environmental stress tolerant plant

活性氧清除酶的基因调控及耐环境胁迫植物的选育

• 批准号: 10460149
• 负责人: TANAKA Kunisuke
• 金额: $ 4.29万
• 依托单位: Kyoto Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10460149/
• 关键词: rice; environmental stress tolerant plant breeding; superoxide dismutase; plastidic; oxygen stress; active oxygen; ascorbate peroxidase; glutathione reductase; 遺伝子発現制御; グルタレドキシン; チオレドキシ; Redox制御; 形質転換タバコ; アスコンビン酸パーオキシターゼ; Fe-SOD; 細胞質型GR; 過

To clarify the gene regulation of active oxygen scavenging enzymes and the repairing enzyes that work for repairing mainly of the proteins damaged by the active oxygen species in plant has been the aim of this project. Results obtained through this project is expected to be useful for plant breeding of tolerant plants to environmental stresses. Through fulfillment of this project the gene regulation mechanisms of SODs became clearer. Isolation and analysis of the genes those are working cooperatively with SOD genesto scavenge active oxygen molecules and to repair damages of the molecules have been achieved.Rice Fe-SOD cDNA, APXs, malic dehydrogenase cDNA and rice gutathione reductase gene were structurally analyzed and the detailed gene regulations have been also studied.Plant cytosolic SOD, glutaredoxin, thioredoxin were revealed to have a highly homologous sequence composed of 77 base pairs. This specific sequence is considered to be a regulatory region for the promoter of these genes that work for Redox responses. We further found in this 77 base region an AT rich 28 base pairs region which we named as COREII. This COREII was revealed to be a cisfactor and recognized that this factor interacts specifically with some nucleoproteins.We produced several transgenic tobacco plants that over express SOD(Spinach) and/or APX(Arabidopsis) genes. Thses transgenic plants showed tolerance to cold and drought stresses. This fact indicates that SOD and APX genes are useful to breed environmental stress tolerant plants.

阐明植物体内活性氧清除酶和修复酶的基因调控是本课题的目标。本计画之研究结果可望对培育耐环境胁迫之植物有所助益。通过本项目的完成，对SOD的基因调控机制有了进一步的认识。对水稻Fe-SOD cDNA、APXs、苹果酸脱氢酶cDNA和谷氨酰胺还原酶基因进行了结构分析和详细的基因调控研究，并对植物胞质SOD、谷氧还蛋白、谷胱甘肽还原酶、谷胱甘肽还原酶、谷胱甘肽过氧化物酶、硫氧还蛋白具有由77个碱基对组成的高度同源序列。该特定序列被认为是这些基因的启动子的调节区，这些基因对氧化还原反应起作用。我们进一步在这77个碱基的区域中发现了一个富含AT的28个碱基对的区域，我们将其命名为COREII。该COREII是一个顺式因子，与一些核蛋白特异性相互作用。我们获得了几株过量表达SOD（Spinach）和/或APX（Arabidopsis）基因的转基因烟草。这些转基因植株表现出对低温和干旱胁迫的耐受性。这一事实表明，SOD和APX基因是有用的，以培育耐环境胁迫的植物。

项目成果

Jun'ichi Urano: "Molecular cloning and characterization of a rice dehydroascorbate reductase"FEBS Letters. 466. 107-111 (2000)

Junichi Urano：“水稻脱氢抗坏血酸还原酶的分子克隆和表征”FEBS Letters。

H.Kaminaka: "Gene cloningand expression of cytosolic glutathione reductase in rice(Oryza sativa L.)" Plant Cell Physiol.39(12). 1269-1280 (1998)

H.Kaminaka：“水稻胞浆谷胱甘肽还原酶的基因克隆和表达（Oryza sativa L.）”植物细胞生理学.39（12）。

Shigeto Morita: "Induction of rice cytosolic asorbate peroxidase mRNA by oxidative stress ; the involvement of hydrogen peroxide in oxedative stress signalling"Plant Cell Physiol.. 40. 417-422 (1999)

Shigeto Morita：“通过氧化应激诱导水稻细胞溶质山梨酸过氧化物酶 mRNA；过氧化氢参与氧化应激信号”植物细胞生理学.. 40. 417-422 (1999)

Hironori Kaminaka: "Gene cloning and expression of cytosolic glutathione reductase. in rice (Oryza sativa L.)"Plant Cell Physiol.. 39. 1269-1280 (1998)

Hironori Kaminaka：“水稻（Oryza sativa L.）中细胞质谷胱甘肽还原酶的基因克隆和表达”植物细胞生理学.. 39. 1269-1280 (1998)

Shigeto Morita and Kunisuke Tanaka: "Detoxification of active oxygen species and tolerance in plants exposed to air pollutants and CO_2."Air Pollution and Biotechnology in Plants (K Omasa et al eds.),Springer-Verlag Tokyo.

Shigeto Morita 和 Kunisuke Tanaka：“暴露于空气污染物和 CO_2 的植物中活性氧的解毒和耐受性。”空气污染和植物生物技术（K Omasa 等编辑），Springer-Verlag 东京。