US-France Cooperative Research: Structural and Functional Study of Thioredoxins

美法合作研究：硫氧还蛋白的结构和功能研究

• 批准号: 8914522
• 负责人: Bob Buchanan
• 金额: $ 1.18万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-02-01 至 1993-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8914522&HistoricalAwards=false
• 关键词: US; France; Cooperative; Research; Structural

This award will support collaborate research between Dr. Bob Buchanan, University of California at Berkeley and Dr. M. Miginiac- Maslow, University of Paris-Sud, Orsay. The overall goal of the project is to relate the structure and function of the newly identified extrachloroplastic thioredoxin h of leaves and other plant tissues and of the m-type thioredoxins present in the green eukaryotic alga Chlamydomonas reinhardtii. Theoredoxins are low- molecular-weight proteins that are widely distributed in nature. In pursuing the proposed work, the investigators will identify the structural domains involved in the interaction of thioredoxins with different target enzymes. They plan to perform site-directed mutagenesis experiments to confer different activities (specifici- ties) to the higher plant and algal thioredoxins and convert them to either a more bacterial or animal-like character. These results will provide insight into the structural features that determine the specificity of thioredoxins, not only of plants, but also of animals and bacteria. Research from this study will add fundamental new knowledge to several fields, including biochemistry, medicine and biotechnology. The complementary interests and expertise of the Berkeley and Orsay groups, together with their long-standing record of successful scientific cooperation, will help insure the success of the proposed research.

该奖项将支持鲍勃博士和 布坎南，加州大学伯克利分校和M。米吉尼亚克- 马斯洛，南巴黎大学，奥赛。 的总体目标 项目是将新的结构和功能 已确定的叶和其他的叶绿体外硫氧还蛋白h 植物组织和存在于绿色中的m-型硫氧还蛋白 真核生物莱茵衣藻 Theoredoxins是低- 分子量的蛋白质在自然界中广泛分布。 在开展拟议的工作时，调查人员将确定 结构域参与的相互作用硫氧还蛋白与 不同的目标酶。 他们计划进行现场指导 诱变实验赋予不同的活性（特异性， 联系）到高等植物和藻类硫氧还蛋白，并将它们转化为 细菌或动物的特征。 这些 结果将提供深入了解的结构特征， 确定硫氧还蛋白的特异性，不仅是植物， 也包括动物和细菌。 这项研究将增加 基础新知识的几个领域，包括生物化学， 医学和生物技术。 互补的利益和 伯克利和奥赛小组的专业知识，以及他们的 长期以来成功的科学合作记录，将 帮助确保拟议研究的成功。