Genetic and Biochemical Studies of Lignolytic Bacteria

木质素分解细菌的遗传和生化研究

• 批准号: 8816265
• 负责人: T. Kent Kirk
• 金额: $ 1.26万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-02-01 至 1992-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8816265&HistoricalAwards=false
• 关键词: Genetic; Biochemical; Studies; Lignolytic; Bacteria

This award supports cooperative research in forest products research between T. Kent Kirk of the University of Wisconsin at Madison and Jose Vicuna of the Catholic University in Santiago, Chile. They will undertake genetic and biochemical studies of bacteria responsible for lignin biodegradation. Natural bacterial strains will be sought that can metabolize low molecular weight lignin-related substrates. Then the metabolic pathways involved in the degradation of lignin model compounds will be identified and the enzymes which catalyze the cleavage of intermonomeric linkages will be isolated and characterized. Bacterial growth on lignin and their consumption of fungal degradation products will be analyzed. Finally, a genetic library of lignolytic strains will be constructed. This research will continue a successful collaboration between Kirk, who has experience in lignin chemistry and Vicuna, who has isolated several strains of lignin bacteria. Little work has been done in South America on lignin degradation. Yet its different ecological habitats may yield microbial strains unknown in the northern hemisphere. This work allows the U.S. scientist to include bacterial studies in his work and will aid the research component of the Chilean pulp and paper industry. Their different complementary backgrounds and approaches are likely to lead to advances in pulp bleaching, in modifying fiber properties, in converting lignin to useful products and in waste treatment. In so doing the project fulfills the goals of the Science in Developing Countries Program.

该奖项支持威斯康星大学麦迪逊分校的T·肯特·柯克和智利圣地亚哥天主教大学的何塞·维库纳在森林产品研究方面的合作研究。他们将对负责木质素生物降解的细菌进行遗传和生化研究。将寻找能够代谢低分子木质素相关底物的天然细菌菌株。然后，将确定参与木质素模型化合物降解的代谢途径，并分离和鉴定催化单体间键断裂的酶。将分析细菌在木质素上的生长及其对真菌降解产物的消耗。最后，将构建木质素降解菌株的遗传文库。这项研究将继续柯克和维库纳之间的成功合作，柯克在木质素化学方面有经验，维库纳已经分离出几种木质素细菌。在南美，关于木质素降解的研究很少。然而，它不同的生态环境可能会产生北半球未知的微生物菌株。这项工作使这位美国科学家能够在他的工作中包括细菌研究，并将有助于智利纸浆和造纸工业的研究部分。它们不同的互补背景和方法可能会在纸浆漂白、改善纤维性质、将木素转化为有用的产品和废物处理方面取得进展。通过这样做，该项目实现了发展中国家科学计划的目标。