Light Scattering Studies of DNA Dynamics

DNA 动力学的光散射研究

• 批准号: 8816433
• 负责人: Victor Bloomfield
• 金额: $ 16万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-01-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8816433&HistoricalAwards=false
• 关键词: Light; Scattering; Studies; DNA; Dynamics

The aqueous ionic milieu influences long-range intermolecular interactions between DNA molecules, the degree of expansion or condensation of the DNA molecular coil and the short range helical structure. Within each of these domains, there are striking transitions not yet clearly understood on the basis of existing polyelectrolyte theory or nucleic acid chemistry. Dr. Bloomfield's goal is to understand the forces mediating these transitions. Some of the techniques to be used are dynamic laser scattering, small angle X-ray and neutron scattering, UV-visible, CD and laser Raman spectroscopy and chemical probes. Theoretical techniques will also be used. DNA undergoes some striking phase changes. All of these transitions depend strongly on ionic strength as well as on other variables such as polymer concentration, molecular weight, temperature and specific ion effects. Molecular biology has thus far concerned itself primarily with coding- type data. However, at a higher level, the choice of a code which is used (i.e., the DNA) has control mechanisms built into it which are widely believed to involve in part, binding of other control molecules to the DNA, and in part, changes in the DNA conformation. Knowledge of ways in which DNAs are likely to interact must precede some types of understanding of control mechanisms and the experiments proposed here treat precisely this problem.***//

水离子环境影响长程分子间 DNA分子之间的相互作用， 或DNA分子螺旋的缩合， 螺旋结构 在这些领域中， 尚未明确理解的惊人转变， 或核酸化学的基础上。 布卢姆菲尔德博士的目标是了解 这些过渡。 要使用的一些技术是 动态激光散射，小角X射线和中子 散射、紫外-可见、CD和激光拉曼光谱， 化学探针 还将使用理论技术。 DNA经历了一些惊人的相变。 所有这些 跃迁强烈地依赖于离子强度以及 其它变量例如聚合物浓度、分子量 重量、温度和特定离子效应。 分子 迄今为止，生物学主要关注的是编码， 类型数据。 然而，在更高的层次上， 其被使用（即，DNA）具有内置的控制机制 它被广泛认为部分涉及对其他国家的约束， 控制DNA的分子，部分是DNA的变化， 构象 了解DNA有可能 交互必须先于对控制某些类型的理解 这里提出的机制和实验精确地处理 这个问题。**//