Mechanisms of Virus-Mediated Translational Inhibition

病毒介导的翻译抑制机制

• 批准号: 8816918
• 负责人: Victor Chinchar
• 金额: $ 10万
• 依托单位: University of Mississippi Medical Center
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-06-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8816918&HistoricalAwards=false
• 关键词: Mechanisms; Virus; Mediated; Translational; Inhibition

Attempts are being made to determine the mechanism by which frog virus 3 selectively blocks host cell translation. To accomplish this it is planned to identify and isolate the FV3 virion-associated translational inhibitor, to determine the mechanism of FV3 mediated translational shut-off by identifying where in initiation the translational defect occurs, and to determine whether the 5' untranslated region of viral messages controls their translational efficiency. During the past twenty years, the sequence of events and specific components involved in eukaryotes have been determined and the importance of translation control in gene expression has gained general accpetance. However, despite advances in both areas, there remain significant gaps. For example the precise roles for several eukaryotic initiation factors and function(s) of their various subunits is not known. In light of this, it should be apparent that model systems, of which FV3 is one, will be most important in advancing our understanding of translation and translational control. Dr. Chinchar is well qualified to carry out this research.

人们正在尝试确定青蛙病毒3选择性地阻止宿主细胞翻译的机制。为了实现这一点，计划鉴定和分离FV3病毒粒子相关的翻译抑制物，通过识别翻译缺陷发生在起始位置来确定FV3介导的翻译关闭的机制，并确定病毒信息的5‘非翻译区是否控制其翻译效率。在过去的二十年里，人们已经确定了真核生物所涉及的事件顺序和特定成分，并普遍认识到翻译控制在基因表达中的重要性。然而，尽管在这两个领域都取得了进展，但仍然存在重大差距。例如，几个真核启动因子及其各个亚基的功能(S)的确切作用尚不清楚。鉴于此，很明显，模型系统，其中FV3是其中之一，将是最重要的，以促进我们对翻译和翻译控制的理解。钦查尔博士完全有资格开展这项研究。