Translational Control of Euglena LHCPII Synthesis

这项提案着眼于LHCP II合成的调节， 在翻译控制的水平上。 基因组 和裸藻LHCP II的cDNA克隆正在测序， 确定来源于多蛋白的蛋白质的身份 在LHCP II合成过程中发现的中间体。 LHCP II mRNA水平 并测量LHCP II mRNA向多核糖体中的募集， 确定平移控制是否在 翻译开始或结束后。 混合释放和- 逮捕研究被用来确定在多大程度上， 所有四种前LHCPII都是通过内部启动产生的， 多蛋白mRNA。 这些研究的结果将有助于 了解LHCP II的光调节机制 翻译成Euglena 这项建议着眼于蛋白质合成的光调节 光合作用蛋白LHCP Ⅱ在单细胞生物中的作用 裸藻 这表明了一种不寻常的监管模式， 这种蛋白质使得mRNA的合成保持恒定， 光诱导的LHCP II蛋白的增加与 mRNA到蛋白质的翻译水平增加。 的 该提案中概述的研究正在通过以下方式研究该机制： 这种平移控制是由于光 LHCP II在其他光合生物中的调节作用 在mRNA的生产水平，而不是翻译水平。 的结果 这项研究应该提供新的见解， 翻译控制在高等生物中，包括植物和动物。

This proposal is looking at the regulation of synthesis of LHCPII at the level of translational control in Euglena. Both genomic and cDNA clones of Euglena LHCPII are being sequenced to determine the identity of the proteins derived from a polyprotein intermediate found during LHCPII synthesis. LHCPII mRNA levels and the recruitment of LHCPII mRNA into polysomes are measured to determine whether translational control operates at the level of initiation of translation or afterwards. Hybrid-release and - arrest studies are being used to determine the extent to which all four preLHCPIIs are derived through internal initiation of the polyprotein mRNA. The results of these studies will aid in understanding the mechanisms of light regulation of LHCPII translation in Euglena. This proposal looks at the light regulation of protein synthesis of the photosynthetic protein LHCPII in the unicellular organism Euglena. This alga shows an unusual pattern of regulation of this protein such that synthesis of the mRNA is held constant and the light-induced increase in LHCPII protein is associated with an increased level of translation of the mRNA to protein. The research outlined in this proposal is looking at the mechanism by which this translational control is achieved since the light regulation of LHCPII in other photosynthetic organisms operates at the level of mRNA production, not translation. The results of this research should provide new insights into the mechanisms of translational control in higher organisms, both plant and animal.