RUI: Mechanisms and Structural Components Involved in Development of Plants

转录起始是基因调控的主要部位。这项工作的目标是进一步定义和表征一般转录和基因特异性转录的机制(S)。将获得有关转录装置的结构和功能以及发育和/或光调节基因的表达的基本信息。一些小麦转录因子(TF)已经被分离出来。一种转铁蛋白已经被提纯并足迹到DNA启动子上，它似乎是相当于动物TFIIA的植物。将研究转录复合体的形成，以确定组装过程中出现的中间结构。将继续研究开发RNA聚合酶II体外转录系统和完善RNA聚合酶III体外系统。最后，识别特定基因的转录因子的研究正在开始。有必要改良农作物，以增加每英亩产量，并增加这些作物的营养价值。昆虫和微生物的破坏对植物构成了持续的威胁，也需要植物对环境的变异性和极端情况有更强的耐受性。为了培育出更好的植物品种，现在有可能将新的性状引入植物基因库。因此，这些类型的研究非常需要，而阿克曼博士可以用他的专业知识提供帮助。//

Transcription initiation is a primary site for gene regulation. The goal of this work is to further define and characterize the mechanism (s) of general and gene - specific transcription. Basic information will be aquired on the structure and function of the transcription apparatus and on expression of developmentally and/ or light regulated genes. Several wheat transcription factors (TFs) have been isolated. One TF has been purified and footprinted to a DNA promoter, it appears to be the plant equivalent of animal TFIIA. The transcription complex formation will be investigated in order to define the intermediate structures which occur during assembly. Studies will be continued on developing an in vitro RNA polymerase II transcription system and perfecting an RNA polymerase III in vitro system. Lastly, studies are begining on identifying gene-specific TFs. There is a need to improve agricultural crops to yield more ford per - acre and to increase the nutritional value of these crops. Insect and microbial damage pose a constant threat to plants and there is also a need for plant more tolerant of environmental variability and extremes. To develop better plant species it is now possible to introduce new traits into plant gene pools. Thus these types of studies are sorely needed and Dr. Ackerman can help with his expertise.//