RUI: Mechanisms and Structural Components Involved in Development of Plants

RUI：植物发育中涉及的机制和结构组件

• 批准号: 8817275
• 负责人: Steven Ackerman
• 金额: $ 10万
• 依托单位: University of Massachusetts Boston
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-03-01 至 1991-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8817275&HistoricalAwards=false
• 关键词: RUI; Mechanisms; Structural; Components; Involved

Transcription initiation is a primary site for gene regulation. The goal of this work is to further define and characterize the mechanism (s) of general and gene - specific transcription. Basic information will be aquired on the structure and function of the transcription apparatus and on expression of developmentally and/ or light regulated genes. Several wheat transcription factors (TFs) have been isolated. One TF has been purified and footprinted to a DNA promoter, it appears to be the plant equivalent of animal TFIIA. The transcription complex formation will be investigated in order to define the intermediate structures which occur during assembly. Studies will be continued on developing an in vitro RNA polymerase II transcription system and perfecting an RNA polymerase III in vitro system. Lastly, studies are begining on identifying gene-specific TFs. There is a need to improve agricultural crops to yield more ford per - acre and to increase the nutritional value of these crops. Insect and microbial damage pose a constant threat to plants and there is also a need for plant more tolerant of environmental variability and extremes. To develop better plant species it is now possible to introduce new traits into plant gene pools. Thus these types of studies are sorely needed and Dr. Ackerman can help with his expertise.//

转录起始是基因转录的主要位点， 调控 这项工作的目标是进一步界定和 描述一般和基因的机制- 特异转录 基本信息将在 转录的结构和功能 装置和发育和/或 光调节基因。 几种小麦转录因子 (TFs)被隔离了一个TF已被纯化， DNA启动子的足迹，它似乎是植物 相当于动物TFIIA。 转录复合体 将调查形成，以确定 在组装过程中出现的中间结构。 研究 将继续开发体外RNA聚合酶 II转录系统和完善RNA聚合酶III 体外系统 最后，研究开始确定 基因特异性转录因子 需要改善农作物以提高产量 每英亩的福特和增加营养价值 这些作物。 昆虫和微生物的破坏 对植物的威胁，也需要更多的植物 能容忍环境的变化和极端。 到 开发更好的植物品种，现在可以引入 植物基因库中的新性状。 这些类型的 研究是迫切需要的，阿克曼博士可以帮助他的 专业知识。//