RUI: Mechanisms & Structural Components Involved in Development of Plants

芮：机制

• 批准号: 9118667
• 负责人: Steven Ackerman
• 金额: $ 5.4万
• 依托单位: University of Massachusetts Boston
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9118667&HistoricalAwards=false
• 关键词: RUI; Mechanisms; & Structural; Components

The long term goal is to understand gene regulation during development in plants. The area on which the PI has chosen to focus his efforts is regulation of transcription, specially the initiation step. Assembly of the transcription complex is the first step at which regulation can occur. In order to evaluate events during the assembly process it was first necessary to isolate and purify several plant transcription factors (TFs), then to characterize them (wheat TFIIA and TFIID), and to establish a heterologous wheat/human in vitro transcription system using the cauliflower mosaic (CaMV) 35S promoter. Objectives for the next funding period are: 1. Continued biochemical characterization of wheat TFIIA 2. Continued biochemical characterization of wheat TFIID 3. Enzymology of transcription using wheat TFIIA AND wheat TFIID

长期目标是了解基因调控过程中， 植物的发育。 PI选择的区域 他的工作重点是调节转录，特别是 起始步骤。 转录复合体的组装是 第一步，可以进行监管。 为了评价 在组装过程中，首先需要 分离纯化了几种植物转录因子， 然后对它们进行表征（小麦TFIIA和TFIID）， 建立异源小麦/人体外转录体系 系统使用花椰菜花叶病毒（CaMV）35 S启动子。 下一个供资期的目标是： 1. 小麦TFIIA的连续生化特性研究 2. 小麦TFIID的连续生化特性 3. 使用小麦TFIIA和小麦的转录的酶学 TFIID