Regulation and Role of the Lipid-Transporting Protein, Lipophorin, in Insect Diapause

脂质转运蛋白脂蛋白在昆虫滞育中的调节和作用

• 批准号: 8818688
• 负责人: G. Michael Chippendale
• 金额: $ 4万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-06-01 至 1990-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8818688&HistoricalAwards=false
• 关键词: Regulation; Role; Lipid; Transporting; Protein

The aim of this research is to gain an increased understanding about biochemical mechanisms insects use to survive the winter. While many insect species are known to overwinter in a state of diapause (dormancy) our understanding of the biochemical changes that occur during diapause and permit this long-term survival under adverse conditions is limited. Additional knowledge in this area could lead to new biorational approaches for the control of some pest insects. This proposal focuses on the regulation of the larval diapause of the southwestern corn borer, Diatraea grandiosella. Since lipids serve as a major reserve nutrient for survival during its diapause, the role of the lipids-transporting protein, lipophorin, is being examined in diapause larvae. Although lipids are known to be released from the fat body and transported in the hemolymph to sites of utilization during diapause, little is known about how the process of lipid release from the body fat occurs. The synthesis, processing, and release of lipophorin from the fat body of non-diapause and diapause larvae will be compared in a study and should substantially increase knowledge about the relationship between lipophorin and diapause and provide new information about how lipid reserves are sequestered and then mobilized to sustain insects during a prolonged period of dormancy. Ultracentrifugal, electrophoretic, immunochemical, radioisotopic, and electron microscopic techniques will be employed to do the proposed experiments.

这项研究的目的是加深对昆虫用来越冬的生化机制的了解。虽然已知许多昆虫物种在滞育(休眠)状态下越冬，但我们对滞育期间发生的生化变化以及允许这种在不利条件下长期生存的了解有限。在这一领域的更多知识可能导致控制一些害虫的新的生物进化方法。这项建议的重点是西南部玉米螟幼虫滞育的调控。由于脂类是滞育幼虫生存的主要储备营养物质，因此脂蛋白在滞育幼虫中的作用正在研究中。虽然已知脂肪在滞育期间从脂肪体内释放并在血淋巴中运输到利用部位，但对体内脂肪释放脂肪的过程知之甚少。在一项研究中，将对非滞育和滞育幼虫脂肪体中脂蛋白的合成、加工和释放进行比较，这将大大增加对脂蛋白与滞育之间关系的了解，并提供关于脂类储备如何被隔离然后动员以在长时间休眠期间维持昆虫的新信息。超速离心法、电泳法、免疫化学法、放射性同位素和电子显微镜技术将被用来进行拟议的实验。