Regulation of Differential mRNA Stability in Plastids

质体中差异 mRNA 稳定性的调节

• 批准号: 8818965
• 负责人: Wilhelm Gruissem
• 金额: $ 25.33万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1992-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8818965&HistoricalAwards=false
• 关键词: Regulation; Differential; mRNA; Stability; Plastids

The goal of this proposal is to define the molecular mechanisms of this posttranscriptional regulation as part of the long-term objective to understand the control of organelle gene expression and the role of the nuclear genome in this process. Dr. Gruissem will examine in detail the function of inverted repeat (IR) sequences at the 3' end on plastid mRNAs. These IR sequences are ubiquitous, their sequences is conserved between evolutionary divergent plants, and they do not act as efficient transcription terminators, but homogeneous plastid mRNA 3` ends are established by processing. Preliminary experiments suggest that proteins bind to the 3` IR sequences, and their abundance changes during plant development are correlated with differential mRNA accumulation. This RNA: protein interaction is of interest, because it may delinaeate a novel regulatory mechanism in the control of plastid gene expression. This work should provide new results to help fill the gap that currently exists in our understanding of posttranslational regulatory mechanisms. Additionally the studies may potentially lead to new insights into the role of the nuclear genome in the control of organelle expression.

这项提案的目标是确定分子机制 这种转录后调节作为长期的一部分， 目的了解细胞器基因的调控 表达和核基因组在这一过程中的作用。 Gruissem博士将详细研究倒置 在质体mRNA的3'末端的IR重复序列。 这些IR 序列是普遍存在的，它们的序列在 进化分歧的植物，而且它们的效率不高 转录终止子，但同质质体mRNA 3 '末端 是通过加工建立的。 初步实验表明 蛋白质结合到3` IR序列， 植物发育过程中的变化与 差异mRNA积累。 RNA：蛋白质相互作用 是有趣的，因为它可以定义一种新的调节机制， 控制质体基因表达的机制。 这项工作应该提供新的结果，以帮助填补差距， 目前存在于我们对翻译后 监管机制。 此外，研究可能 可能导致对核武器作用的新认识， 基因组控制细胞器的表达。