Molecular Analysis of the rbcS Multigene Family

转录调控是转录调控中的重要控制步骤。 rbcS基因的发育和器官特异性表达 植物中的家庭在植物基因中，rbcS启动子是其中一个 顺式作用DNA序列元件的最佳特征， 交易因素 相比之下，人们对 将监管部分纳入发展 程序. 使用番茄作为模型系统，我们已经表明， 每个rbcS启动子时间和器官特异性活性被 与DNA结合的复杂相互作用的变化相关 具有共享或独特的特定DNA序列的蛋白质 在启动子区域之间。 在番茄果实中， rbcS启动子DNA-蛋白质复合物在转换过程中消失 从年轻到成熟的果实。 该提案将调查 控制发育和器官特异性 DNA-蛋白质相互作用的差异。 该项目将侧重于 五种不同的DNA结合蛋白， 特色 实验提出建立 蛋白质在rbcS转录控制中的功能， 确定蛋白质的表达是如何调节的 不同器官和果实发育过程中。 %%% 这种方法将提供新的和重要的见解， 控制调节DNA结合蛋白的机制 植物发育

Transcriptional regulation is an important control step in the developmental and organ-specific expression of the rbcS gene family in plants. Among plant genes, the rbcS promoter is one of the best characterized for cis-acting DNA sequence elements and transacting factors. In contrast, much less is known about the integration of the regulatory components into the developmental program. Using tomato as a model system, we have shown that the temporal and organ-specific activity of each rbcS promoter is correlated with changes in the complex interaction of DNA-binding proteins with specific DNA sequences that are shared or unique among the promoter regions. In tomato fruit, all but one of the rbcS promoter DNA-protein complexes disappear during the transition from young to ripening fruit. This proposal will investigate the mechanisms that control the developmental and organ-specific differences in DNA-protein interactions. The project will focus on five different DNA-binding proteins with unique binding characteristics. Experiments are presented to establish the function of the proteins in rbcS transcriptional control and to determine how the expression of the proteins is regulated in different organs and during fruit development. %%% This approach will provide novel and important insights into the mechanisms that control regulatory DNA-binding proteins during plant development.