Regulation of Differential mRNA Stability in Plastids

差异mRNA稳定性的调节是一个重要的控制 在质体基因表达中起重要作用。 格鲁塞姆博士已经证明， 核糖体的结合和mRNA的结构是重要的 叶绿体mRNA衰变的决定因素。 反向重复 叶绿体3'非翻译区的IR-RNA序列 mRNA作为mRNA 3'端的顺式调节决定簇发挥作用 加工和稳定性，并与RNA结合蛋白相互作用。 三个RNA结合蛋白已被纯化和测序 蛋白质的基因已经被克隆。 两种RNA结合 已经鉴定并部分纯化了蛋白质， 特异性地与一种mRNA的3' IR-RNA相互作用。 是 建议继续进行RNA结合的功能分析， 蛋白质在叶绿体mRNA代谢和稳定性中的应用 体外重建系统，并通过遗传干扰的 在拟南芥中的基因表达。 生物化学和 分子方法来确定核糖体如何 联合有助于不同的半衰期的叶绿体 mRNA。 %%% 叶绿体的分子机制剖析 mRNA稳定性的实现将提供新的和通用的 细胞器基因表达的核控制信息。 mRNA加工和稳定性的调节是一个重要的 在几乎所有生物体中控制基因表达的机制。 尽管mRNA稳定性控制广泛存在，但我们的 对这种类型的调节的理解仍然是神秘的， 不完整。//

Regulation of differential mRNA stability is an important control step in plastid gene expression. Dr. Gruissem has shown that ribosome association and structures in the mRNA are important determinants for the decay of chloroplast mRNAs. Inverted repeat sequences (IR-RNAs) in the 3' untranslated region of chloroplast mRNAs function as cis-regulatory determinants for mRNA 3' end processing and stability , and interact with RNA-binding proteins. Three of the RNA-binding proteins have been purified and sequenced and the gene for the proteins has been cloned. Two RNA-binding proteins have been identified and partially purified that specifically interact with the 3' IR-RNA of one mRNA. It is proposed to continue the functional analysis of the RNA-binding proteins in chloroplast mRNA metabolism and stability using in vitro reconstituted systems, and by genetic interference of the expression of their genes in Arabidopsis. A biochemical and molecular approach is presented to determine how ribosome association contributes to the different half-lives of chloroplast mRNAs. %%% Dissection of the molecular mechanisms by which chloroplast mRNA stability is accomplished will provide novel and general information about the nuclear control of organelle gene expression. Regulation of mRNA processing and stability is an important mechanism in control of gene expression in virtually all organisms. Despite the wide-spread occurrence of mRNA stability control, our understanding of this type of regulation is still cryptic and incomplete.//