Regulation of Differential mRNA Stability in Plastids

质体中差异 mRNA 稳定性的调节

• 批准号: 9118215
• 负责人: Wilhelm Gruissem
• 金额: $ 17万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 1994-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9118215&HistoricalAwards=false
• 关键词: Regulation; Differential; mRNA; Stability; Plastids

Regulation of differential mRNA stability is an important control step in plastid gene expression. Dr. Gruissem has shown that ribosome association and structures in the mRNA are important determinants for the decay of chloroplast mRNAs. Inverted repeat sequences (IR-RNAs) in the 3' untranslated region of chloroplast mRNAs function as cis-regulatory determinants for mRNA 3' end processing and stability , and interact with RNA-binding proteins. Three of the RNA-binding proteins have been purified and sequenced and the gene for the proteins has been cloned. Two RNA-binding proteins have been identified and partially purified that specifically interact with the 3' IR-RNA of one mRNA. It is proposed to continue the functional analysis of the RNA-binding proteins in chloroplast mRNA metabolism and stability using in vitro reconstituted systems, and by genetic interference of the expression of their genes in Arabidopsis. A biochemical and molecular approach is presented to determine how ribosome association contributes to the different half-lives of chloroplast mRNAs. %%% Dissection of the molecular mechanisms by which chloroplast mRNA stability is accomplished will provide novel and general information about the nuclear control of organelle gene expression. Regulation of mRNA processing and stability is an important mechanism in control of gene expression in virtually all organisms. Despite the wide-spread occurrence of mRNA stability control, our understanding of this type of regulation is still cryptic and incomplete.//

差异 mRNA 稳定性的调节是质体基因表达的重要控制步骤。 Gruissem 博士表明，mRNA 中的核糖体关联和结构是叶绿体 mRNA 衰变的重要决定因素。 叶绿体 mRNA 3' 非翻译区中的反向重复序列 (IR-RNA) 作为 mRNA 3' 末端加工和稳定性的顺式调节决定因素，并与 RNA 结合蛋白相互作用。 三种 RNA 结合蛋白已被纯化并测序，并且这些蛋白的基因已被克隆。 已鉴定并部分纯化了两种 RNA 结合蛋白，它们与一种 mRNA 的 3' IR-RNA 特异性相互作用。 建议使用体外重建系统，并通过对拟南芥中其基因表达的遗传干扰，继续对叶绿体 mRNA 代谢和稳定性中的 RNA 结合蛋白进行功能分析。 提出了一种生化和分子方法来确定核糖体关联如何影响叶绿体 mRNA 的不同半衰期。 %%% 对实现叶绿体 mRNA 稳定性的分子机制的剖析将提供有关细胞器基因表达的核控制的新颖且一般的信息。 mRNA 加工和稳定性的调节是几乎所有生物体中控制基因表达的重要机制。 尽管 mRNA 稳定性控制广泛存在，但我们对此类调节的理解仍然是神秘且不完整的。//