Genes for Photosystems Components in Cyanobacteria and Cyanelles

蓝细菌和蓝藻中光系统成分的基因

• 批准号: 8818997
• 负责人: Donald Bryant
• 金额: $ 23.84万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1993-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8818997&HistoricalAwards=false
• 关键词: Genes; Photosystems; Components; Cyanobacteria; Cyanelles

Studies are proposed which allow the continued characterization of the Photosystem I (PS I) and Photosystem II (PS II) reaction centers via molecular genetics. The roles of several recently cloned genes, including psaC, psaD, psaE, and psbH, will be pursued by interposon and/or site-directed mutagenesis. The primary goal of the proposed work is to establish methods and strains which will allow a detailed analysis of PS I structure and function in Synechococcus sp. PCC 7002. Special attention will be given to the low-molecular-mass polypeptides associated with the PS I and PS II reaction centers. Additional work on the role(s) of light and nutrients in the regulation of cyanobacterial gene expression will be pursued by transcript analyses and gene fusion methods. Finally, we will continue to explore the phylogenetic relationships among cyanobacteria, cyanelles, and chloroplasts.

建议进行研究，以便继续表征 光系统I（PS I）和光系统II（PS II）反应的 通过分子遗传学。 最近几个角色 克隆的基因，包括psaC，psaD，psaE和psbH，将被 通过插入子和/或定点诱变进行。 的 拟议工作的主要目标是建立方法和 菌株，这将允许PS I结构的详细分析 并在聚球藻PCC 7002中起作用。 特别注意 将给予低分子量多肽相关 与PS I和PS II反应中心连接。 的额外工作 光和营养素在调节 蓝藻基因表达将通过转录本进行追踪， 分析和基因融合方法。 最后，我们将继续 探索蓝藻之间的系统发育关系， 蓝色素和叶绿体。