Photosystem I: Biogenesis, Broken Symmetry, and Hydrogenase Chimeras

光系统 I：生物发生、对称性破缺和氢化酶嵌合体

• 批准号: 0519743
• 负责人: Donald Bryant
• 金额: --
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0519743&HistoricalAwards=false
• 关键词: Photosystem; Biogenesis; Broken; Symmetry; Hydrogenase

This project represents a highly integrated, comprehensive research and training program between the laboratories of Co-PIs Donald A. Bryant and John H. Golbeck. This research program includes the application of transposon mutagenesis and proteomic methods to the identification of biochemical factors that govern the bioassembly of membrane proteins, the application of magnetic resonance spectroscopy to probe the conformational dynamics encountered during the bioassembly of membrane proteins, the application of directed mutagenesis and spectroscopic techniques to discover factors that contribute to the redox potentials of organic electron transfer cofactors, and the construction of Photosystem I-hydrogenase chimeras. These aims are within reach because research in Photosystem I has achieved a level of sophistication that allows problems of global importance to biochemistry to be addressed. The studies in this project will be greatly facilitated by the recently completed genome sequence of the euryhaline, unicellular cyanobacterium Synechococcus sp. PCC 7002. The availability of this genome sequence, as well as a nearly complete transposon mutation library, will be exploited in searching for factors involved in the biogenesis of Photosystem I. This project will have a significant and broad impact by providing unique opportunities to train undergraduate students, graduate students, and postdoctoral fellows in disciplines ranging from microbial physiology, molecular biology, genomics, biochemistry, to biophysics. This research will also present excellent opportunities for collaborative research between the PI's students and postdoctoral scientists with foreign scientists who are acknowledged experts in their fields. The PI and the co-PI will continue their efforts to involve faculty and undergraduates from predominantly undergraduate institutions in their research.

该项目代表了一个高度集成的，全面的研究和培训计划之间的实验室合作PI唐纳德A。Bryant和John H.戈尔贝克该研究计划包括转座子诱变和蛋白质组学方法的应用，以确定生物化学因素，管理膜蛋白的生物组装，应用磁共振光谱探测膜蛋白的生物组装过程中遇到的构象动力学，应用定向诱变和光谱技术发现影响有机电子氧化还原电位的因素转移辅因子和构建光系统I-氢化酶嵌合体。这些目标是可以实现的，因为光系统I的研究已经达到了一个复杂的水平，可以解决全球重要的生物化学问题。最近完成的广盐性单细胞蓝藻聚球藻7002的基因组测序将大大促进本项目的研究。这个基因组序列的可用性，以及一个几乎完整的转座子突变库，将被利用在寻找参与光系统I的生物发生的因素。该项目将通过提供独特的机会来培养本科生，研究生和博士后研究员，从微生物生理学，分子生物学，基因组学，生物化学到生物物理学等学科，产生重大而广泛的影响。这项研究也将为PI的学生和博士后科学家与在各自领域公认的专家的外国科学家之间的合作研究提供极好的机会。PI和co-PI将继续努力让来自主要本科院校的教师和本科生参与他们的研究。