Chloroplast-Derived Nuclear DNA: Prevalance and Mechanisms of Transposition

叶绿体衍生的核 DNA：转座的普遍性和机制

• 批准号: 8819210
• 负责人: Eran Pichersky
• 金额: $ 13.3万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8819210&HistoricalAwards=false
• 关键词: Chloroplast; Derived; Nuclear; DNA; Prevalance

We have recently identified chloroplast-derived DNA sequences in an intron of a tomato nuclear gene that we had cloned. Although it had been demonstrated, by hybridization experiments, that the nuclear genomes of many plants contain sequences derived from the chloroplast genome, this is the first example of the physical isolation of such integrates. Our analysis of the integrates and of the integration sites suggests the involvement of homologous recombination mechanisms in the transposition events. In this proposal we outline experiments designed to physically isolate and characterize many chloroplast DNA integrates in the tomato nuclear genome. The date obtained will be used to test the hypothesis that homologous recombination is involved in the transposition of such chloroplast sequences into the nuclear genome. The presence of DNA sequences integrated into the cellular genome which originated in the organelle genomes has been abundantly documented. However, little or nothing is known about the mechanism(s) involved. The results of this study should provide insight into those processes.

我们最近在我们克隆的番茄核基因的一个内含子中鉴定了叶绿体衍生的DNA序列。虽然通过杂交实验已经证明，许多植物的核基因组中包含来自叶绿体基因组的序列，但这是物理分离这种整合的第一个例子。我们对整合和整合位点的分析表明，同源重组机制参与了转座事件。在这项提案中，我们概述了旨在物理分离和鉴定整合到番茄核基因组中的许多叶绿体DNA的实验。获得的数据将被用来检验这样的叶绿体序列转座到核基因组中涉及同源重组的假设。DNA序列整合到细胞基因组中的存在，起源于细胞器基因组，已有大量文献记载。然而，对所涉及的机制(S)知之甚少或一无所知。这项研究的结果应该会对这些过程有深入的了解。