Structure of a Molluscan Neuropeptide Hormone Receptor

软体动物神经肽激素受体的结构

• 批准号: 8819838
• 负责人: Gregg Nagle
• 金额: $ 5万
• 依托单位: University of Texas Medical Branch at Galveston
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-03-01 至 1990-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8819838&HistoricalAwards=false
• 关键词: Structure; Molluscan; Neuropeptide; Hormone; Receptor

Model systems using invertebrates have provided insightful and innovative new information towards elucidating more complex neural events in mammals. Dr. Nagle is using the control of egg- laying behavior in the marine mollusc Aplysia to understand the cellular and molecular bases of neuroendocrine function. The neurosecretory bag cells that synthesize and release a novel neuropeptide egg-laying hormone (ELH) are model neuroendocrine cells that have been used extensively to elucidate how neural cells function. His research program is aimed at isolating and characterizing a membrane receptor protein which, in the intact animal, binds the ELH and initiates a complex series of cellular and behavioral events that culminate in egg laying and reproductive functions. The gene that encodes this behaviorally important receptor protein will be cloned and sequenced. With this knowledge, functional questions about how a hormonal signal may be transduced by neural and non-neural cells can be examined. Thus, understanding the expression of the peptide receptor in this simple behavioral system should enhance our understanding and lead to generalities about receptor expression in neuroendocrine functions.

使用无脊椎动物的模型系统为阐明哺乳动物中更复杂的神经事件提供了有洞察力和创新性的新信息。Nagle博士正在利用对海洋软体动物海兔产卵行为的控制来了解神经内分泌功能的细胞和分子基础。神经分泌袋细胞合成和释放一种新的神经肽产卵激素(ELH)，是一种模型神经内分泌细胞，已被广泛用于阐明神经细胞的功能。他的研究项目旨在分离和鉴定一种膜受体蛋白，这种蛋白在完好的动物体内与ELH结合，并启动一系列复杂的细胞和行为事件，最终导致产卵和生殖功能。编码这种行为上重要的受体蛋白的基因将被克隆和测序。有了这些知识，关于神经和非神经细胞如何传递荷尔蒙信号的功能性问题就可以被研究出来。因此，了解多肽受体在这个简单的行为系统中的表达应该会增强我们的理解，并导致关于神经内分泌功能中受体表达的一般性认识。