Cell Biology of Prohormone Processing in Identified Peptidergic Neurons

已识别的肽能神经元中激素原加工的细胞生物学

• 批准号: 9511773
• 负责人: Gregg Nagle
• 金额: $ 19.5万
• 依托单位: University of Texas Medical Branch at Galveston
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-06-15 至 2000-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9511773&HistoricalAwards=false
• 关键词: Cell; Biology; Prohormone; Processing; Identified

Nagle 9511773 Small protein molecules or peptides, play an important role in communication between cells in the brain. The synthesis of these peptides is a complex task involving the excision of the peptide from a larger precursor molecule by specific enzymes. Moreover, these peptides must be compartmentalized or packaged in the brain in such away that they can be released upon stimulation of the brain cell. To accomplish this release, the larger precursor molecules and the enzymes to process the precusor must be co-stored so the information transmitting peptide can be cut from the larger molecule and released between cells. However, how this packaging of material is accomplished remains unknown. Dr. Nagle will use a well-established system to investigate how the enzymes responsible for cleaving these peptides from their precursors is accomplished. It is anticipated that these studies will also identify specific domains in the enzymes responsible for targeting the peptide-containing precursors and enzymes to the appropriate secretory apparatus. These studies are of fundamental importance in understanding the transfer of information between cells. The findings from these studies are applicable to cells in the brain, as well as peripheral tissues. More importantly, however, these studies provide information on the biochemical basis by which information is transmitted between cells in the nervous system or the basis of the way the brain functions.

Nagle 9511773 小的蛋白质分子或肽在大脑细胞之间的通信中起着重要作用。 这些肽的合成是一项复杂的任务，涉及通过特定的酶从较大的前体分子中切除肽。 此外，这些肽必须在脑中被区室化或包装，使得它们可以在刺激脑细胞时释放。 为了完成这种释放，较大的前体分子和加工前体的酶必须共同储存，这样信息传递肽就可以从较大的分子中切割出来，并在细胞之间释放。 然而，这种材料的包装是如何完成的仍然是未知的。 Nagle博士将使用一个完善的系统来研究负责将这些肽从其前体中切割出来的酶是如何完成的。 预计这些研究还将鉴定负责将含肽前体和酶靶向至适当分泌器的酶中的特定结构域。 这些研究对于理解细胞之间的信息传递具有重要意义。 这些研究的结果适用于大脑细胞以及外周组织。 然而，更重要的是，这些研究提供了关于神经系统细胞之间信息传递的生化基础或大脑功能的基础的信息。