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Single Cells Make Seashells on the Seashore: using scRNAseq to uncover the role of haemocytes in molluscan biomineralization

单细胞在海边制造贝壳：使用 scRNAseq 揭示血细胞在软体动物生物矿化中的作用

基本信息

批准号：
2885049
负责人：
金额：
--
依托单位：
University of Aberdeen
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2023
资助国家：
英国
起止时间：
2023 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2885049
关键词：
Single Cells Make Seashells Seashore

项目摘要

How do molluscs build their shells? Shellfish farmers want to grow disease-resistant animals with strong shells, climate change scientists are concerned about the fate of calcifiers in warmer more acidic oceans, and biotechnology industries mine shells for bioactive compounds and material properties. Despite hundreds of years of scientific curiosity, our understanding on the cellular basis of seashell production is incomplete. In 2004, it was hypothesised that immune cells, called haemocytes, make intracellular calcium crystals and deposit them onto the shell. More recently, methods such as transcriptomics and proteomics revealed haemocytes have a molecular signature that could indicate biomineralisation functions. But a complete molluscan immune cell development (haematopoiesis) description and functional tests to confirm roles in biomineralisation are still lacking. The broad question this project will address is: do immune cells, called haemocytes, participate in mollusc shell secretion? To disentangle the potential role of haemocytes in biomineralization, from their known functions in immunity, the project will use the cutting-edge technique single-cell RNA-sequencing, in two mollusc model systems: Crepidula fornicata and Crassostrea gigas. The project will interrogate scRNAseq datasets to uncover the cell lineage and developmental events that give rise to haemocytes before conducting experiments to empirically test their function.

软体动物是如何造壳的？贝类养殖者希望培育出具有强壮外壳的抗病动物，气候变化科学家担心在酸性更强的温暖海洋中钙化物的命运，生物技术行业从贝壳中开采生物活性化合物和材料特性。尽管数百年来对科学的好奇，我们对贝壳生产的细胞基础的理解是不完整的。2004年，有人提出一种假设，即被称为血细胞的免疫细胞在细胞内形成钙晶体，并将其沉积在外壳上。最近，转录组学和蛋白质组学等方法揭示了血细胞具有可以指示生物矿化功能的分子特征。但是完整的软体动物免疫细胞发育（造血）描述和功能测试来确认生物矿化的作用仍然缺乏。这个项目将解决的广泛问题是：被称为血细胞的免疫细胞是否参与软体动物的贝壳分泌？为了将血细胞在生物矿化中的潜在作用从它们已知的免疫功能中分离出来，该项目将在两种软体动物模型系统中使用尖端技术单细胞rna测序：fornicata Crepidula和长牡蛎（Crassostrea gigas）。该项目将查询scRNAseq数据集，以揭示产生血细胞的细胞谱系和发育事件，然后进行实验以经验性地测试其功能。