Transmission Electron Microscope for the University of Maine

作为中央研究机构，电子显微镜 缅因州大学的实验室是一个广泛的资源， 各种项目在生物以及物理和 化学科学 利用该设施的研究项目 呼吁一种新的透射显微镜配备了一个共着丝粒 测角器阶段;该提案要求为这样一个 显微镜 已确定22名调查人员， 代表了一系列的应用，该显微镜将 应用;五个这些研究人员，与NSF支持 项目被确定为主要用户。 具体项目 例如，这项建议要求， 体视学分析，以澄清线粒体的作用， 高氧和缺氧条件下以及在适应生活条件下， 低温，以表征昼夜变化， 光感受器，并量化酸雨胁迫对 植物和动物组织 超微结构免疫细胞化学 将用于一些项目中识别细胞外基质 低等无脊椎动物的组成部分，以定位酶， 植物/真菌协会，并表征木材的酶 降解 在系统生物学中， 超微结构特征将用于阐明进化 硅藻、高等植物和低等植物之间的关系 无脊椎动物 在分子生物学和病毒学中的应用 包括原生生物染色体外DNA特征， 遗传物质和结构的表征 Mycovirus.

As a central research facility, the Electron Microscopy Laboratory of the University of Maine is a resource for a wide variety of projects in the biological as well as physical and chemical sciences. Research projects making use of this facility call for a new transmission microscope equipped with a eucentric goniometer stage; this proposal requests funding for such a microscope. Twenty-two investigators have been identified to represent a range of applications to which this microscope will be applied; five fo these investigators, with NSF-supported projects are identified as major users. Projects specified in this proposal call, for example, for ultrastructural stereological analysis to clarify the roles of mitochondria in hyperoxic and hypoxic conditions and in acclimation to life at low temperatures, to characterize diurnal changes in photoreceptors, and to quantify effects of acid-rain stress in plant and animal tissues. Ultrastructural immunocytochemistry will be used in some projects to identify extracellular matrix components of lower invertebrates, to localize enzymes in plant/fungal associations, and to characterize enzymes of wood degradation. In systematic biology, phylogenetic analysis of ultrastructural characters will be used to elucidate evolutionary relationships in diatoms, higher plants, and lower invertebrates. Applications in molecular biology and virology include characterization of extrachromosomal DNA in a protist and characterization of genetic material and structure of a mycovirus.//