200keV, Energy Filtered, Intermediate-High Voltage Transmission Electron Microscope(IVEM)"

项目摘要/摘要 这份申请概述了我们购买新的200keV、能量过滤、中高电压的资金请求 电压透射电子显微镜(IVEM)(Thermo-Fisher/FEI Talos F200C G2)。这台仪器将会是 完全是加利福尼亚州拉霍亚研究台地(以及许多研究机构)所独有的，并被引入 使用尖端(和互补)方法实现能量过滤、多标签传输的生产 电子显微镜(如ColorEM)和高通量三维电子显微镜断层扫描(3D EM) 厚厚的环氧树脂包埋的细胞和组织样本(非低温)。采购这台显微镜将解决 两个迫切需求：1)它将直接支持双方对这些成像方法/技术不断增长的需求 当地和全国分布的NIH调查人员，目前是国家显微镜中心和 成像研究(NCMIR)；和2)它将只提供以前对尖端方法的生产访问 可在高端、专门的平台上使用，旨在支持ColorEM等方法的开发(实现 国家卫生研究院的目标是降低使用门槛，促进更广泛的传播和影响)。因此，它还将 推动这些关键技术的民主化。指明的新文书将在 与即将取代的JEOL 3200 IVEM相比，成本要低得多， JEOL很快就无法维护了。新工具还简化了安装，对空间的要求更低， 允许更多的NIH研究人员采用它所支持的方法。该平台将自动化并设置- 供社区轻松访问，并得到电子显微镜专家团队的支持，标本 准备和数据处理、分析和可视化。它的环境还将包括(和直接制作 使用)圣地亚哥超级计算机的新型高性能计算和数据存储基础设施 中心及其附属的国家研究平台(NRP)。

PROJECT SUMMARY / ABSTRACT This application outlines our request for funding to procure a new 200keV, energy filtered, intermediate-high voltage transmission electron microscope (IVEM) (Thermo-Fisher/FEI Talos F200C G2). This instrument will be completely unique to the La Jolla, CA research Mesa (and it’s many research organizations) and bring into production use cutting edge (and complementary) methods for energy filter enabled, multiple label transmission electron microscopy (e.g., ColorEM) and high throughput 3D electron microscopic tomography (3D EM) of semi- thick epoxy-embedded samples of cells and tissues (non-Cryo). Procurement of this microscope will address two pressing needs: 1) it will directly support growing demand for these imaging methods/technologies by both local and Nationally distributed NIH investigators, currently users of the National Center for Microscopy and Imaging Research (NCMIR); and 2) it will provide production access to cutting-edge methods previously only available on high-end, specialized platforms built to enable development of methods like ColorEM, (fulfilling a goal of NIH to lower barriers to the use and to promote broader dissemination and impact). As such, it will also propel the democratization of these key technologies. The specified new instrument will be procured at a significantly lower cost, compared to the instrument it will replace, a 20 year old custom-built JEOL 3200 IVEM, soon not maintainable by JEOL. The new tool also simplifies installation, with more modest room requirements, allowing adoption of the methods it enables by more NIH researchers. The platform will be automated and set- up for easy access by the community and be supported by a team of experts in electron microscopy, specimen preparation and data processing, analysis, and visualization. Its environment will also include (and make direct use) of novel high-performance computing and data storage infrastructure of the San Diego Supercomputer Center and its affiliated National Research Platform (NRP).