Developmental Biology and Genetics of the Maize Leaf

玉米叶的发育生物学和遗传学

• 批准号: 8820790
• 负责人: Michael Freeling
• 金额: $ 6.04万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-03-01 至 1989-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8820790&HistoricalAwards=false
• 关键词: Developmental; Biology; Genetics; Maize; Leaf

The core of this study involves the characterization of leaf developmental mutants in maize. Two have been cloned via transposon-tagging. The questions addressed in this proposal are: 1.Are the leaf development mutants under study altered in cellular age-identity, as hypothesized, or in position-identity? Problems of developmental time-keeping are especially approachable in plants. 2. Does the animal concept of developmental compartments apply to plants? Dr. Freeling will address this question using a dominant dwarf in genetic mosaic studies. 3. Are the provascular cells the primary inducers of other leaf cell fates? Is the epidermis an information "blank sheet" until induced to differentiate into particular cell types? Dr. Freeling will approach these two related question using detailed vein lineage maps, periclinal chimeric analyses, and mutants that fail to anastomose intermediate veins. %%% These studies will establish the maize leaf as a powerful developmental system amenable to mutant analysis as well as for cell biological, and embryological studies. The approach to the mutants at these levels will provide a context for the on-going studies on the molecular descriptions of developmental gene regulation.

这项研究的核心涉及玉米叶片发育突变体的表征。其中两个已通过转座子标记被克隆。该提案解决的问题是： 1.所研究的叶片发育突变体是否如假设的那样在细胞年龄同一性上发生了改变，或者在位置同一性上发生了改变？ 发育时间保持问题在植物中尤其容易解决。 2. 动物的发育区室概念是否适用于植物？弗里林博士将在遗传镶嵌研究中利用显性矮人来解决这个问题。 3. 前维管细胞是其他叶细胞命运的主要诱导物吗？在被诱导分化成特定细胞类型之前，表皮是一张信息“白纸”吗？ Freeling 博士将使用详细的静脉谱系图、临床嵌合分析和无法吻合中间静脉的突变体来解决这两个问题。 %%% 这些研究将把玉米叶确立为一个强大的发育系统，适合突变体分析以及细胞生物学和胚胎学研究。在这些水平上研究突变体的方法将为正在进行的发育基因调控的分子描述研究提供背景。