Developmental Biology and Genetics of the Maize Leaf

本研究的核心是叶的表征 玉米的发育突变体其中两个已经通过 转座子标记本提案涉及的问题是： 1.所研究的叶片发育突变体是否在 细胞的年龄认同，如假设的那样，还是位置认同？ 发展中的计时问题尤其突出， 在植物中可接近。2.动物的概念 植物的发育区室Freeling医生会 在遗传嵌合体中使用显性侏儒来解决这个问题 问题研究3.原血管细胞是 其他叶细胞的命运表皮是一个信息“空白 “片”，直到诱导分化成特定的细胞类型？ Freeling博士将使用以下方法来处理这两个相关的问题： 详细的静脉谱系图，平周嵌合体分析， 不能使中间脉变粗的突变体。 %%% 这些研究将建立玉米叶作为一个强大的 适合突变分析的发育系统以及用于 细胞生物学和胚胎学研究。的途径 这些水平的突变体将为正在进行的 发育基因的分子描述研究 调控

The core of this study involves the characterization of leaf developmental mutants in maize. Two have been cloned via transposon-tagging. The questions addressed in this proposal are: 1.Are the leaf development mutants under study altered in cellular age-identity, as hypothesized, or in position-identity? Problems of developmental time-keeping are especially approachable in plants. 2. Does the animal concept of developmental compartments apply to plants? Dr. Freeling will address this question using a dominant dwarf in genetic mosaic studies. 3. Are the provascular cells the primary inducers of other leaf cell fates? Is the epidermis an information "blank sheet" until induced to differentiate into particular cell types? Dr. Freeling will approach these two related question using detailed vein lineage maps, periclinal chimeric analyses, and mutants that fail to anastomose intermediate veins. %%% These studies will establish the maize leaf as a powerful developmental system amenable to mutant analysis as well as for cell biological, and embryological studies. The approach to the mutants at these levels will provide a context for the on-going studies on the molecular descriptions of developmental gene regulation.