Ultra-low freezer and lab washer for a molecular cell biology and developmental genetics laboratory

适用于分子细胞生物学和发育遗传学实验室的超低温冰箱和实验室清洗机

• 批准号: 345072-2007
• 负责人: Staveley, Brian
• 金额: $ 0.85万
• 依托单位: Memorial University of Newfoundland
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=326041
• 关键词: Ultra; low; freezer; lab; washer

Standard laboratory equipment items, specifically an ultra-low freezer and a laboratory washer, are required for our laboratory's experiments into cell death, cell survival and growth. Over recent years, the process of programmed cell death has become very well understood. To actively counteract cell death, survival mechanisms involving the insulin receptor (and molecules activated by it) function to help cells live and grow. In our laboratory, we study such processes in the common fruit fly, an ideal model organism in which to study the fundamental aspects of cell biology. A few years ago, we demonstrated that reduced levels of a protein activated by the insulin receptor result in cell death in a developing multicellular organism. Recently we found that another factor is key in the interpretation of low levels of insulin receptor activity and acts to reduce growth and alter metabolism in fruit flies. Most importantly, we have found that this factor may provide a short-term survival mechanism when nutrients are unavailable, and thus may have an important role in the sensing of nutrition. As cell death plays a role in degeneration of neurons, we have started to investigate the cellular basis of neurodegeneration and its prevention. Currently, we are investigating other crucial components of these mechanisms that control cell growth and cell death. With the recent advances derived from the human and other genome projects, discoveries in model organisms promise to offer information important to the survival and growth of humans. This line of inquiry promises to advance our knowledge relating to many conditions suffered by Canadians, such as diabetes, obesity, and neurodegenerative diseases including Parkinson's Disease and Huntington's Disease. In summary, our continued research into the molecular cell biology of fruit flies will provide a great opportunity to explore the fundamental mechanisms that control the delicate balance between life and death.

标准实验室设备项目，特别是超低温冷冻机和实验室清洗机，是我们实验室进行细胞死亡，细胞存活和生长实验所必需的。近年来，程序性细胞死亡的过程已经得到了很好的理解。为了积极对抗细胞死亡，涉及胰岛素受体（及其激活的分子）的生存机制有助于细胞存活和生长。在我们的实验室中，我们在普通果蝇中研究这些过程，果蝇是研究细胞生物学基本方面的理想模式生物。几年前，我们证明了在发育中的多细胞生物中，胰岛素受体激活的蛋白质水平降低会导致细胞死亡。最近，我们发现另一个因素是解释低水平胰岛素受体活性的关键，并在果蝇中起到降低生长和改变代谢的作用。最重要的是，我们已经发现，这个因素可能会提供一个短期的生存机制时，营养素是不可用的，因此可能有一个重要的作用，在传感的营养。由于细胞死亡在神经元变性中起作用，我们已经开始研究神经变性的细胞基础及其预防。目前，我们正在研究控制细胞生长和细胞死亡的这些机制的其他关键组成部分。随着人类和其他基因组计划的最新进展，模式生物的发现有望提供对人类生存和成长至关重要的信息。这条调查线有望推进我们对加拿大人遭受的许多疾病的了解，如糖尿病，肥胖症和神经退行性疾病，包括帕金森病和亨廷顿病。总之，我们对果蝇分子细胞生物学的持续研究将为探索控制生与死之间微妙平衡的基本机制提供一个很好的机会。