US-UK Cooperative Research: Identification of Ligands for Carbohydrate-Recognition Domains

该奖项将支持生物化学领域的合作研究 在哥伦比亚大学的库尔特·德里卡默博士和腾·菲兹博士之间， 医学研究理事会临床研究中心，哈罗， 英格兰米德尔塞克斯 该项目的目标是研究 某些动物的碳水化合物结合特异性 哺乳动物凝集素蛋白质。 一种常见的碳水化合物识别结构域（CRD）已被发现， 在具有不同生物学功能的蛋白质中发现。 蛋白 含有这些同源结构域的蛋白质包括血清甘露糖结合蛋白 蛋白质和肝膜受体，介导 去唾液酸糖蛋白的内吞作用。 基于一级结构 信息，CRD已被预测并在其他蛋白质中发现， 如肺表面活性物质脱辅基蛋白和核心蛋白 软骨蛋白多糖 用于产生这些的表达系统 土培大肠杆菌中的结构域正在由 _______________ 美国的首席研究员 在这个项目中， 提出了与Ten Feizi博士的合作，他的研究小组 已经开发出一种新糖脂的印迹方法， 研究人员确定详细的糖结合 各种CRD的特异性，也将用于 鉴定所述结构域的内源性配体。 通过组合 Drickamer博士的蛋白质和分子生物学专业知识 实验室与Feizi博士小组的碳水化合物技术， 将有可能启动一种强有力的新分析方法， 碳水化合物识别的生物学作用。 Drickamer博士 是哺乳动物凝集素分子生物学的领导者， 并证明了他能够使用 用于表达碳水化合物的重组DNA方法 这些蛋白质的结合域。

This award will support collaborative research in biochemistry between Dr. Kurt Drickamer, Columbia University and Dr. Ten Feizi, Medical Research Council Clinical Research Center, Harrow, Middlesex, England. The objective of the project is to study the detailed carbohydrate binding specificities of certain animal proteins called mammalian lectins. A type of common carbohydrate-recognition domain (CRD) has been identified in proteins with diverse biological functions. Proteins containing these homologous domains include serum mannose binding proteins and the hepatic membrane receptor, which mediates endocytosis of asialoglycoproteins. Based on primary structure information, CRDs have been predicted and found in other proteins, such as pulmonary surfactant apoprotein and core protein of cartilage proteoglycan. Expression systems for producing these domains in Eschericia coli are being developed in the laboratory of _______________ the principal investigator in the United States. In this project, a collaboration is proposed with Dr. Ten Feizi; his research group has developed a method of blotting neoglycolipids which will allow the investigators to determine the detailed saccharide-binding specificity of the various CRDs and which will also be used to identify endogenous ligands for the domains. By combining the protein and molecular biology expertise of Dr. Drickamer's laboratory with the carbohydrate technology of Dr. Feizi's group, it will be possible to initiate a powerful new approach to analysis of the biological role of carbohydrate recognition. Dr. Drickamer is a leader in the molecular biology of mammalian lectins and related proteins and has demonstrated his ability to use recombinant DNA approaches for expression of the carbohydrate binding domain of these proteins.