3-D Computer Graphics and Analysis of Microscopic Images of Biological Structures

先进的交互式串行重建系统将 将制定和实施方案， 厚切片数据的导出和可视化 电子显微镜倾斜系列 该奖项将提供 这些努力的基础。 San的设施 迭戈超级计算机中心将用于实施和 探索计算机三维图形方法 数据的重建和可视化表示为 一系列的平面或横截面通过一个三 次元介质 计算机软件工具将 不仅对生物学家， 气象学家、海洋学家和地质学家， 探索数据的三维分布方面 以层或地层的形式收集。 将程序 正在一个热情的泰坦和一个硅图形上实现， IRIS工作站。 电子显微镜图像被 分析以获得大量的三维信息 生物标本的结构。 的方法 采用二维图像的断层摄影重建 并利用先前的发展， 倾斜序列和三维能量分布。 的 计算机的进步将应用于三维 细胞图像的重建， 光学显微镜，分子和大分子的图像 组件以及神经解剖标测系统。

An advanced interactive serial reconstruction system will be developed, and programs will be implemented for the derivation and visualization of data from thick section electron microscopy tilt series. This award will provide an initial basis for these efforts. The facilities of the San Diego Supercomputer Center will be utilized to implement and explore computer graphic methods for the three dimensional reconstruction and visualization of data represented as a series of planes or cross sections through a three dimensional medium. The computer software tools will be available not only for biologists but also for meteorologists, oceanographers and geologists who wish to explore three dimensional distributional aspects of data collected in the form of layers or strata. The programs are being implemented on an Ardent Titan and a Silicon Graphics IRIS workstation. The electron microscopic images are being analyzed to obtain substantial three dimensional information about the structure of biological specimens. The methods employ tomographic reconstruction of two dimensional images and exploit prior developments for registration of digitized tilt series and three dimensional energy distribution. The computer advances will be applied to three dimensional reconstruction of cellular images from both electron and light microscopy, to images of molecular and macromolecular assemblies, and to neuroanatomical mapping systems.