3-D Computer Graphics and Analysis of Microscopic Images of Biological Structures

生物结构显微图像的 3D 计算机图形学和分析

• 批准号: 8822633
• 负责人: Mark Ellisman
• 金额: $ 4.49万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1992-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8822633&HistoricalAwards=false
• 关键词: Computer; Graphics; Analysis; Microscopic; Images

An advanced interactive serial reconstruction system will be developed, and programs will be implemented for the derivation and visualization of data from thick section electron microscopy tilt series. This award will provide an initial basis for these efforts. The facilities of the San Diego Supercomputer Center will be utilized to implement and explore computer graphic methods for the three dimensional reconstruction and visualization of data represented as a series of planes or cross sections through a three dimensional medium. The computer software tools will be available not only for biologists but also for meteorologists, oceanographers and geologists who wish to explore three dimensional distributional aspects of data collected in the form of layers or strata. The programs are being implemented on an Ardent Titan and a Silicon Graphics IRIS workstation. The electron microscopic images are being analyzed to obtain substantial three dimensional information about the structure of biological specimens. The methods employ tomographic reconstruction of two dimensional images and exploit prior developments for registration of digitized tilt series and three dimensional energy distribution. The computer advances will be applied to three dimensional reconstruction of cellular images from both electron and light microscopy, to images of molecular and macromolecular assemblies, and to neuroanatomical mapping systems.

将开发一个先进的交互式串行重建系统，并将实施程序以从厚部分电子显微镜倾斜系列的数据推导和可视化数据。 该奖项将为这些努力提供初始依据。 圣地亚哥超级计算机中心的设施将用于实现和探索计算机图形方法，用于通过三维介质作为一系列平面或横截面表示的三维重建和可视化数据。 计算机软件工具将不仅适用于生物学家，还适用于希望探索以层或层级形式收集的数据的三维分布方面的气象学家，海洋学家和地质学家。 这些程序正在激进的泰坦和硅图形虹膜工作站上实施。 正在分析电子显微镜图像，以获得有关生物标本结构的大量三维信息。 该方法采用层析成像重建，对两个维图像进行重建，并利用先前的发展进行数字化倾斜序列的注册和三维能量分布。 计算机的进步将应用于从电子和光学显微镜，分子和大分子组件的图像以及神经解剖学映射系统的三维重建。