Presidential Young Investigator Award: Design and Strategies for Optimizing Bioprocesses

总统青年研究员奖：优化生物过程的设计和策略

• 批准号: 8857719
• 负责人: Dhinakar Kompala
• 金额: $ 34.2万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-01 至 1993-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8857719&HistoricalAwards=false
• 关键词: Presidential; Young; Investigator; Award; Design

This research covers a variety of important problems in the field of biotechnology; however, the major emphasis is focussed on studies of recombinant bacterial cultures, the elimination of plasmid instability, and the overproduction of recombinant products. Both batch-fed and continuous cultures are being examined with the ultimate goal of achieving optimal process control of the systems. Affinity cell separation techniques will be used to increase the longevity of recombinant bacterial cultures. It is hoped that this can be accomplished by the selective removal of plasmid-free segregants. Also, technolgy for maximizing hybridoma cell production of monoclonal antibodies and the use of high-voltage electric pulses for the electrofusion of animal cells are being investigated. The primary thrust of this work is to improve bioreactor performance for the manufacture of economic bioproducts. The result that is desired is that many biologicals that can only be produced in micro quantities today will be manufactured in kilogram or greater quantities in the near future. This achievement would make bioproducts available at affordable cost and, therefore, contribute to an improved standard of living for the general populous.

这项研究涵盖了该领域的各种重要问题， 生物技术;然而，主要重点是集中在研究 重组细菌培养物，质粒不稳定性的消除， 以及重组产物的过度生产。 分批进料和 连续培养的最终目标是 实现系统的最佳过程控制。 亲和细胞分离技术将用于提高细胞的生物学活性。 重组细菌培养物的寿命。 希望这可以 通过选择性去除无质粒的分离物来实现。 此外，用于使单克隆抗体的杂交瘤细胞生产最大化的技术 抗体和使用高压电脉冲 正在研究动物细胞的电融合。 这项工作的主要目的是提高生物反应器的性能， 经济生物产品的生产。 期望的结果是 许多只能以微量生产的生物制品 今天将在近期以公斤或更大的数量生产 未来 这一成就将使生物产品以负担得起的价格提供 成本，因此有助于提高生活水平， 一般人口。