Enhancing Productivity and Protein Quality in Perfusion Cultures

提高灌注培养的生产力和蛋白质质量

• 批准号: 0541119
• 负责人: Dhinakar Kompala
• 金额: --
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0541119&HistoricalAwards=false
• 关键词: Enhancing; Productivity; Protein; Quality; Perfusion

0541119KompalaThe therapeutic efficacy of a biopharmaceutical protein is extremely sensitive to the production conditions because they influence post-translation modifications. Therefore, any new concept for production of such proteins used in humans requires thorough characterization of its impact on the integrity of the product. The proposed study will investigate the author's hypothesis that slow growing perfusion cultures produce more therapeutically relevant glycoproteins compared to those produced in faster growing batch cultures. This proposal addresses an issue that has profound consequences from the practical perspective of advancing the development of an important new class of therapeutic agents (i.e., recombinant glycoproteins). Success in this area will have a significant financial payoff. In addition, from the perspective of "advancing knowledge" this proposal also has as one of its aims the goal of better understanding the underlying biological processes that determine glycosylation patterns.

0541119Kompala生物制药蛋白质的治疗效果对生产条件极其敏感，因为它们影响翻译后修饰。因此，任何用于人类的此类蛋白质生产的新概念都需要彻底表征其对产品完整性的影响。拟议的研究将调查作者的假设，即与快速生长的分批培养物相比，缓慢生长的灌注培养物产生更多的治疗相关糖蛋白。 该提议解决了从推进一类重要的新治疗剂（即，重组糖蛋白）。这方面的成功将带来巨大的经济回报。此外，从“推进知识”的角度来看，该提案的目标之一也是更好地理解决定糖基化模式的潜在生物学过程。