Engineering CHO Cells to Achieve a High-Level Inverse-Growth-Associated Production of Secreted Glycoproteins in High Cell Density Cultures

改造 CHO 细胞以在高细胞密度培养物中实现分泌糖蛋白的高水平逆生长相关生产

• 批准号: 9817249
• 负责人: Dhinakar Kompala
• 金额: $ 38.79万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9817249&HistoricalAwards=false
• 关键词: Engineering; CHO; Cells; Achieve; Level

The objective of this project is to increase the efficiency of cell culture systems by; (a) genetic manipulation of the cells; and (b) utilization of a cell retention system. Genetic manipulation utilizing an AML promoter specific to the G1-phase adds to the benefits of increasing the specific production rate at lower growth rates. Under these conditions, cells can be cultured at low media exchange rates and a higher titer can be achieved. Utilization of a cell retention system increases the cell density and allows a higher volumetric productivity. The CHO expression system considered in this proposal is extensively used in industry and the results from this research will be very applicable to industrial manufacturing.

本项目的目的是通过以下方式提高细胞培养系统的效率：（a）细胞的遗传操作;（B）利用细胞保留系统。 利用对G1期特异的AML启动子的遗传操作增加了在较低生长速率下增加比生产速率的益处。 在这些条件下，细胞可以在低培养基交换率下培养，并且可以获得更高的滴度。 细胞保留系统的使用增加了细胞密度并允许更高的体积生产率。 本提案中考虑的CHO表达系统在工业中广泛使用，并且本研究的结果将非常适用于工业制造。