Presidential Young Investigator Award: Modified and Linked Metalloproteins

总统青年研究员奖：修饰和连接金属蛋白

• 批准号: 8858387
• 负责人: Nenad Kostic
• 金额: $ 28.07万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1994-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8858387&HistoricalAwards=false
• 关键词: Presidential; Young; Investigator; Award; Modified

This Presidential Young Investigator Award from the Inorganic, Bioinorganic and Organometallic Chemistry Program is in the area of bioinorganic chemistry. Metal ions will be used in a variety of ways to probe the structure and function of biomolecules. Noninvasive transition-metal probes will be used to map the binding surfaces of metalloproteins by selective tagging, and the effects of ancillary ligands on the selective recognition between metal complexes and biological macromolecules will be investigated. Intraprotein electron transfer in metalloproteins will be explored through a systematic study of the kinetics and mechanism of electron transfer reactions between active sites of metalloproteins and excited states of inorganic chromophores attached selectively to the protein backbone. Mononuclear and polynuclear transition metal complexes will be used to cross-link metalloproteins to produce potential multielectron redox catalysts. The cleavage of proteins and peptides by metal aquo complexes will be explored, with special focus on the selective delivery of hydrolytic agents to peptide bonds which are not cleavable by common enzymes. Finally, Pt-195 NMR spectroscopy will be developed as a new technique for the study of stereodynamics. This project is expected to provide versatile new tools for the investigation of the function of metalloproteins.

无机、生物无机和有机金属化学项目颁发的总统青年研究员奖属于生物无机化学领域。金属离子将以多种方式用于探测生物分子的结构和功能。 非侵入性过渡金属探针将用于通过选择性标记来绘制金属蛋白的结合表面图谱，并研究辅助配体对金属配合物和生物大分子之间选择性识别的影响。 通过系统研究金属蛋白活性位点与选择性附着在蛋白质主链上的无机发色团的激发态之间的电子转移反应的动力学和机制，将探索金属蛋白中的蛋白内电子转移。 单核和多核过渡金属配合物将用于交联金属蛋白以产生潜在的多电子氧化还原催化剂。 将探索金属水合复合物对蛋白质和肽的裂解，特别关注将水解剂选择性递送至普通酶无法裂解的肽键。 最后，Pt-195 NMR 光谱将被开发为立体动力学研究的新技术。 该项目预计将为金属蛋白功能的研究提供多功能的新工具。