Palladium-containing Artificial Peptidases

含钯人工肽酶

• 批准号: 9404971
• 负责人: Nenad Kostic
• 金额: $ 24.75万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-01 至 1996-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9404971&HistoricalAwards=false
• 关键词: Palladium; containing; Artificial; Peptidases

9404971 Kostic Iowa State Univ. Dr. Nenad Kostic, Chemistry Department, Iowa State University, is supported by the Inorganic, Bioinorganic, and Organometallic Chemistry Program for the development of Pd(II) complexes as new inorganic chemical reagents for selective cleavage of proteins. Kostic has discovered that regioselective hydrolysis of peptides and proteins is promoted by Pt(II) and Pd(II) complexes. In this project the mechanism of this cleavage will be studied and the role of the metal ion analyzed. The information obtained in these studies will be used to increase the selectivity of the cleavage reactions and to design reagents which will perform the cleavage catalytically. Selective cleavage of large proteins into well defined smaller parts is important in analytical biochemistry and biotechnology. In current practice a group of enzymes known as proteases are used to perform this operation. However, the proteases do not cleave folded proteins and they generate many relatively short pieces of the protein, whereas large segments are often desired. This research is aimed at overcoming these problems with a new type of reagent based on the metals, platinum and palladium. Already this project has shown promise in biopharmaceutical research, where the selective cleavage of a protein has led to a potential commercial product.

9404971科斯提克爱荷华州立大学爱荷华州立大学化学系Nenad Kostic博士得到了无机化学、生物无机化学和金属有机化学计划的支持，致力于开发Pd(II)络合物作为选择性切割蛋白质的新无机化学试剂。Kostic发现，铂(II)和钯(II)络合物促进了多肽和蛋白质的区域选择性水解。在本项目中，将研究这种解理的机理，并分析金属离子的作用。在这些研究中获得的信息将被用来增加切割反应的选择性，并设计将催化切割的试剂。在分析生物化学和生物技术中，将大的蛋白质选择性地切割成定义明确的小部分是很重要的。在目前的实践中，一组被称为蛋白酶的酶被用来执行这一操作。然而，这些蛋白水解酶不能裂解折叠的蛋白质，它们会产生许多相对较短的蛋白质片段，而人们通常需要较大的片段。本研究旨在用一种基于金属铂和钯的新型试剂来克服这些问题。这个项目已经在生物制药研究中显示出了希望，在生物制药研究中，蛋白质的选择性切割已经导致了一种潜在的商业产品。