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Protein-Protein Orientation and Dynamics in Electron-Transfer Reactions

Protein-Protein Orientation and Dynamics in Electron-Transfer Reactions
电子转移反应中的蛋白质-蛋白质取向和动力学
批准号:
9222741
负责人:
Nenad Kostic
金额:
$29.1万
依托单位:
依托单位国家:
美国
项目类别:
Continuing Grant
财政年份:
1993
资助国家:
美国
项目状态:
已结题
起止时间:
1993-08-01 至 1997-01-31

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中文摘要
翻译
本研究的目的是了解静电(非共价)蛋白/蛋白复合物中电子转移的机制。将利用完全表征的金属蛋白细胞色素c (cyt)和质体青素(pc)来研究最佳反应速率和特异性的分子基础。基于该实验室最近的成功,提出了一个详细的计划,结合分子生物学(pc突变),计算化学(电子转移路径分析)和动力学(激光闪光光解),以实现以下目标:(1)确定cyt/pc复合物的反应取向;(2)确定血红素到Cu位点的显性转移路径;(3)研究远距离电子耦合的变化如何控制电子转移速率;(4)确定cyt/pc配合物的结构重排速率,建立“门控”的动力学要求;(5)探讨蛋白质/蛋白质界面构象波动对电子转移的重要性。这项研究很重要,因为金属蛋白的电子转移反应对各种生物过程至关重要。这些反应与动物代谢和植物光合作用有关。在分子水平上对电子转移有更深入的了解,对于更好地理解活细胞中化学能的起源和转化是必要的。***
英文摘要
9222741 Kostic The objective of this research is to understand the mechanism of electron transfer in electrostatic (noncovalent) protein/protein complexes. The molecular basis for the optimal reaction rate and specificity will be studied with the fully-characterized metalloproteins cytochrome c (cyt) and plastocyanin (pc). Building upon this laboratory's recent successes, a detailed plan is proposed that combines molecular biology (mutation of pc), computational chemistry (analysis of electron-transfer paths), and kinetics (laser flash photolysis) in order to achieve the following: (1) determine reactive orientations of cyt/pc complexes; (2) pinpoint dominant transfer paths from the heme to the Cu site; (3) examine how variations in distant electronic couplings controls the rate of electron transfer; (4) determine the rate of structural rearrangement of cyt/pc complexes and establish kinetic requirements for "gating"; and (5) explore the importance for electron transfer of conformational fluctuations at the protein/protein interface. %%% This research is important because electron-transfer reactions of metalloproteins are essential to various biological processes. These reactions are involved in animal metabolism and plant photosynthesis. A deeper understanding of electron transfer, at the molecular level, is necessary for a better understanding of the origin and transformations of chemical energy in a living cell. ***
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Metal Complex Bioconjugates as Artificial Enzymes
  • 批准号:
    0316868
  • 项目类别:
    Continuing Grant
  • 资助金额:
    $41.4万
  • 财政年份:
    2003
  • 负责人:
    Nenad Kostic
  • 依托单位:
GOALI: Metal Complexes as Artificial Peptidases
  • 批准号:
    9816522
  • 项目类别:
    Standard Grant
  • 资助金额:
    $30.7万
  • 财政年份:
    1999
  • 负责人:
    Nenad Kostic
  • 依托单位:
Dynamic Processes in Metalloprotein Complexes
  • 批准号:
    9808392
  • 项目类别:
    Standard Grant
  • 资助金额:
    $30.0万
  • 财政年份:
    1998
  • 负责人:
    Nenad Kostic
  • 依托单位:
Palladium-containing Artificial Peptidases
  • 批准号:
    9404971
  • 项目类别:
    Continuing Grant
  • 资助金额:
    $24.75万
  • 财政年份:
    1994
  • 负责人:
    Nenad Kostic
  • 依托单位:
海外基金