Pharmaceutical Production by Immobilized Cells

摘要叙述描述：本提案支持康涅狄格大学的Robert W.Coughlin博士和扎加齐格大学的A.Halim M.El-Sayed博士和Wafaa M.Mahmoud博士的合作，埃及。每名埃及科学家都持有和平奖学金，这将使他们能够在康涅狄格大学与考夫林博士共事约五个月。项目资金将允许(1)延长他们的逗留时间，如果为他们的研究进展拨款，(2)允许他们返回埃及工作，然后第二次返回康涅狄格州继续项目工作，(3)使私人投资者能够访问扎加齐格大学，与他们合作第三个阶段。这项合作正在使用固定的微生物细胞来生产右旋糖苷，一种血浆扩张剂，以及一种广泛使用的治疗药物麻黄素。正在研究新的固定化方法、新的固定化载体和新的生产技术。赛义德博士正在致力于从蔗糖中生产右旋糖苷，而马哈茂德博士将致力于治疗药物L麻黄碱的生产。这两名埃及科学家拟议的研究工作将把生产两种重要药物的技术引入埃及，并可能导致在该国发展这两种药物的生产，使原本可能无法获得这些有效药物的埃及人民受益。范围：这项建议将汇集两国大学在微生物转化和酶和细胞固定化领域工作的科学研究科学家。科学技能和研究方法的发展将从美国转移到埃及，首先是扎加齐格大学，如果成功，然后是埃及当地生产的两种重要药物，一种是血浆扩张器，另一种是治疗药物。这项研究将需要开发新的一般知识和程序，以固定细胞和设计生物催化剂和相关的生物反应器。这项建议实现了发展中国家科学计划通过国际合作交流和转让科学知识的目标。

ABSTRACT NARRATIVE Description: This proposal supports the collaboration of Dr. Robert W. Coughlin, University of Connecticut and Drs. A. Halim M.M. El-Sayed and Wafaa M. Mahmoud of Zagazig University, Zagazig, Egypt. Each Egyptian scientist is a holder of a Peace Fellowship, which will enable them to spend about five months at the University of Connecticut working with the Dr. Coughlin. Project funding will allow (1) an extension of their stay, if appropriated for the progress of their research, (2) permit them to return to Egypt to work and then to return to Connecticut a second time to continue project work and (3) enable the P.I. to visit Zagazig University to work with them a third period. The collaboration is using immobilized microbial cells for producing dextran, a blood plasma expander, and ephedrine, a widely used therapeutic drug. New immobilization method, new immobilization carriers and new production technologies are being investigated. Dr El-Sayed is working on production of dextran from sucrose, while Dr. Mahmoud will work on production of the therapeutic drug, L-ephedrine. The proposed research efforts of the two Egyptian scientists will introduce technology for the production of two important drugs into Egypt and could lead to the development of their production in that country to benefit the Egyptian population that might not otherwise have access to these efficacious drugs. Scope: This proposal will bring together scientific research scientists from the universities of two countries working in the area of microbial transformations and immobilization of enzymes and cells. The development of scientific skills and research methods will be translated from the U.S. to Egypt, first to Zagazig University and if successful, then to local Egyptian production of two important drugs, one a plasma expander and the second, a therapeutic. This research will require the development of new general knowledge and procedures for immobilizing cells and designing biocatalysts and associated bioreactors. This proposal fulfills objectives of the Science in Developing Countries Program in its exchange and transfer of scientific knowledge through a international collaboration.