Plant Genes Expressed Early in Development of Medicago Truncatula Root Nodules

蒺藜苜蓿根瘤发育早期植物基因的表达

• 批准号: 8903709
• 负责人: James Cooper
• 金额: $ 22.5万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-01-01 至 1993-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8903709&HistoricalAwards=false
• 关键词: Plant; Genes; Expressed; Early; Development

Nitrogen-fixing root nodules result from an endosymbiotic association between Rhizobium bacteria and specific legume host plants. Dr. Cooper will utilize cellular and molecular genetic approaches to investigate early events in the development of root nodules in the Rizobium meliloti-Medicago truncatula symbiosis. The specific goals of this proposal are: 1) to clone and characterize the nodulin genes expressed early in nodule development, with particular attention paid to (hydroxy)proline- rich cell wall glycoprotein genes; 2) to examine the spatial and temporal patterns of early symbiotic gene expression at both the protein and nucleic acid levels; and 3) to use reporter gene fusions to identify the cis-acting regulatory sequences responsible for the observed patterns of gene expression, and to begin characterizing the signal molecules used by Rhizobium to regulate plant development. Results of these studies will provide new tools for the molecular genetic dissection of nodule development and plant-microbe interactions. %%% The mechanisms regulating the association of nitrogen fixing bacteria with the roots of compatible plants is a problem of interest both as a basic science question of how organisms interact and affect each other and a problem with great agricultural economic and ecological interest. Plants which are susceptible to this mode of interaction do not require the same addition of nitrogenous fertilizer for their growth.

固氮根瘤是由一种内共生菌 根瘤菌与豆科植物特异寄主的关系 植物库珀博士将利用细胞和分子遗传学 研究根发育早期事件的方法 苜蓿根瘤菌与蒺藜苜蓿共生。 这项建议的具体目标是：1）克隆和 表征根瘤中早期表达的拟南芥素基因 发展，特别注意（羟基）脯氨酸- 丰富的细胞壁糖蛋白基因; 2）检测空间和 早期共生基因表达的时间模式， 蛋白质和核酸水平;和3）使用报告基因 融合以鉴定顺式作用调节序列 负责观察到的基因表达模式， 开始表征根瘤菌用来 调节植物发育。这些研究的结果将提供 结核分子遗传学解剖的新工具 发展和植物-微生物相互作用。 %%% 固氮作用的调控机制 细菌与兼容植物的根是一个问题， 这既是一个基础科学问题， 相互作用和影响， 农业经济和生态利益。的植物 易受这种相互作用模式影响的人不需要同样的 增加氮肥以促进其生长。