Ecology of Methane Oxidizing Microbes

甲烷的微生物氧化是甲烷生物降解的重要组成部分。 全球碳循环 然而，微生物（甲烷氧化菌）， 生态系统中的甲烷氧化尚未确定。 碳 使用酶的测定在淡水样品中的代谢， 独特的甲基营养菌的不同群体将研究， 以部分表征优势微生物区系。 将对样品中的甲醇脱氢酶进行表征。 用于进一步表征存在的甲基营养菌。 氧化甲烷和氨的微生物将通过 表征编码甲烷单加氧酶和甲醇的基因 脱氢酶和每种生物的近似生物量将是 使用DNA-DNA和DNA-RNA杂交反应测量， 以及通过对特定酶的测定。 量化和 这些细菌的鉴定将提供以下信息： 他们对全球温室效应的贡献。

Methane oxidation by microbes is an important part of the global carbon cycle. However, the microbes (methanotrophs) that oxidize methane in ecosystems have not been identified. Carbon metabolism in freshwater samples using assays of enzymes that are unique to different groups of methylotrophs will be studied in order to partially characterize the dominant microflora. Characterization of methanol dehydrogenases in samples will be utilized to further characterize the methylotrophs present. Microbes that oxidize methane and ammonia will be identified by characterizing genes encoding methane monooxygenase and methanol dehyrogenase and the approximate biomass of each organism will be measured using DNA-DNA and DNA-RNA hybridization reactions as well as by assays for spceific enzymes. The quantification and indentification of these bacteria will provide information on their contribution to the global greenhouse effect.