Multi-User Resource for X-Ray Data Collection at Ambient andCryogenic Temperatures

为了将三维晶体结构测定所需的x射线数据收集的宝贵仪器资源恢复到“最先进”的状态，建议将现有的单区域探测器系统升级为传统的双探测器配置，并配备电动探测器测角仪。并为低温下的数据收集提供仪器。对该设施的这些改进将大大提高其在蛋白质结构-功能关系领域的广泛项目中的用途，包括结构确定和分析参与蛋白质合成（翻译）和中间代谢的酶、血红蛋白协同作用机制、艾滋病病毒基因产物和呼吸氧运输。

To restore a valuable instrumental resource for X-ray data collection required for three-dimensional crystal structure determination to "state-of-the-art" status, it is proposed to upgrade an existing, single area detector system to the conventional dual-detector configuration with motorized detector goniometry, and to provide instrumentation for data collection at cryogenic temperatures. These modifications to the facility will greatly enhance its use in a broad spectrum of projects in the area of protein structure-function relationships, including structure determinations and analyses of enzymes involved in protein synthesis (translation) and intermediary metabolism, the mechanism of cooperativity in hemoglobin, gene products from the AIDS virus, and respiratory oxygen transport.