MRI: Acquisition of a Flow Cytometer, a Multi-User Resource at Appalachian State University

这项授予阿巴拉契亚州立大学的奖励是购买一台流式细胞仪，以支持在整个校园内扩大研究、培训和教育活动。除了促进各种PI的研究活动外，流式细胞仪还将用于培训本科生和硕士研究生完成独立研究和论文项目。该仪器还将用于教授免疫学实验室，并可能被纳入现有和新的本科生和研究生课程，为来自多个学科的传统和代表性不足的学生提供服务，如细胞生物学和生物化学。除了作为阿巴拉契亚州立大学所有部门的多用户设备外，该仪器还将用于周边阿巴拉契亚社区的推广目的，包括在夏季创业和未来科学家和医生等既定暑期项目中培训高中生。使用流式细胞仪完成的项目是至关重要的艺术和科学学院和大学的更广泛的努力，以提高研究能力和科学素养，通过提供一个设备齐全，综合的研究和教育环境，我们的学生。该奖项支持的研究还将通过制定在室温下稳定抗体的策略来推进大学对可持续发展的承诺，减少储存和运输通常所需的制冷对环境的影响。此外，作为该奖项的一部分提出的项目有可能为减少营养不良和健康差距提供更广泛的见解。该奖项将支持多名教师在有关免疫学，生物化学，生物能量学，内分泌学和遗传学的项目在校园。具体来说，我们将首先研究锌和铁的缺乏如何影响细胞在暴露于各种寄生虫和病毒时的生长和分裂方式。我们还将研究天然植物产品如何弥补营养缺乏并促进细胞生长。第二个项目将研究水泡性口炎病毒（VSV）如何与宿主免疫细胞相互作用，这与了解如何设计更有效，更有针对性的抗病毒治疗方法有关。第三个项目涉及筛选一种缓步动物蛋白质所需的条件，以稳定其他蛋白质，使它们可以在不冷藏的情况下运输。其他项目将研究炎症，细胞周期和发育，以及植物种群遗传学。所有这些项目都将加强阿巴拉契亚州立大学教师之间的研究合作，并为扩大综合和跨学科的研究关系提供机会。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估来支持。

This award to Appalachian State University is to purchase a flow cytometer to support the expansion of research, training and educational activities across campus. In addition to facilitating research activities by various PIs, the flow cytometer will be used to train undergraduate and Master's level graduate students in completing their independent research and thesis projects. The instrument will also be used to teach immunology labs and may be incorporated in existing and new undergraduate and graduate courses serving traditional and underrepresented students from multiple disciplines, such as cell biology and biochemistry. In addition to serving as a multi-user equipment for all departments at Appalachian State University, the instrument will be utilized for outreach purposes for surrounding Appalachian communities, including training high school students in established summer programs such as Summer Ventures and Future Scientists and Doctors. The projects completed using the flow cytometer are critical to the broader efforts of the College of Arts and Sciences and the university to improve research capabilities and scientific literacy by providing a well-equipped, integrated research and educational environment to our students. Research supported by this award will also advance the University’s commitment to sustainability by developing strategies to stabilize antibodies at room temperature, reducing the environmental impact of refrigeration typically required for storage and transportation. Furthermore, the projects proposed as part of this award have the potential to offer broader insights into reducing malnutrition and health disparities. This award will support multiple faculty members across campus in projects relating to immunology, biochemistry, bioenergetics, endocrinology, and genetics. Specifically, we will first look at how deficiency in zinc and iron influence the way cells grow and divide when exposed to various parasites and viruses. We will also investigate how natural plant products can compensate for nutrient deficiency and promote cell growth. The second project will investigate how vesicular stomatitis virus (VSV) interacts with host immune cells which has relevance for understanding how to design more effective, and targeted, anti-viral therapeutics. The third project involves screening conditions required for a protein from tardigrades to stabilize other proteins so that they can be transported without refrigeration. Other projects will investigate inflammation, cell cycle and development, and plant population genetics. All these projects will enhance collaborations in research among faculty at Appalachian State University and offer opportunities for expansion of integrative and interdisciplinary research relationships.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.