Collaborative Project: Calcium in the Early Development of Hydrozoans

合作项目：水螅动物早期发育中的钙

• 批准号: 8904377
• 负责人: Ellis Ridgway
• 金额: $ 9.39万
• 依托单位: Virginia Commonwealth University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-15 至 1993-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8904377&HistoricalAwards=false
• 关键词: Collaborative; Project; Calcium; Early; Development

Drs. Ridgway and Freeman have recently discovered that the eggs and embryos of several species of hydrozoans contain endogenous calcium indicators similar to aequorin. Because the calcium indicators are natural, exist in high concentration, and emit light specifically in the presence of calcium, these preparations offer unique opportunities for studying the role of calcium in early development. They propose to investigate the mechanism that controls the appearance of calcium channels in the membrane at first cleavage in these organisms. These channels could play a role in establishing the anterior-posterior axis of the developing embryo. They will determine where and why these channels appear by using image intensification and image enhancement techniques in conjunction with microinjection of heterochronic cytoplasm. They have discovered that potassium depolarization of cleavage stage embryos leads to a bright flash which is due in part to calcium release from an internal store. They will investigate the mechanism that couples the calcium entry through the calcium channels to the calcium release internally. They will study the propagation of four sorts of calcium waves: 1. those due to IP3 diffusion in unfertilized eggs, 2. those that may occur at fertilization, 3. those due to depolarization of cleavage stage embryos, and 4. those accompanying metamorphosis. %%% The importance of calcium as a regulator of a number of cellular processes has been established. The question remains as to how is the level of calcium itself controlled. Drs. Ridgway and Freeman have developed a powerful model system in which to address this question. It exploits a naturally occurring luminescent protein found in hydrozoan embryos which emits light in the presence of calcium.

里奇韦博士和弗里曼博士最近发现 几种水螅虫的胚胎含有内源性 钙指示剂类似于水母发光蛋白。因为钙 指示剂是天然的，存在于高浓度中， 特别是在钙的存在下，这些制剂 提供了独特的机会来研究钙在 早期发展。他们建议调查的机制， 控制膜中钙通道的出现， 第一次分裂。这些频道可以播放 在建立前后轴的作用 胚胎发育他们将决定在哪里和为什么这些 通道出现使用图像增强和图像 增强技术结合显微注射 异时细胞质他们发现钾 卵裂期胚胎的去极化导致明亮的闪光 这部分是由于钙从内部储存中释放。 他们将研究将钙离子 通过钙通道进入钙释放 在内部他们将研究四种类型的传播 钙波：1.那些由于IP3扩散在未受精 鸡蛋，2.这些可能发生在受精，3。那些由于 卵裂期胚胎的去极化，和4.那些 伴随着变形。 %%% 钙作为一种调节剂的重要性， 过程已经建立。问题仍然是， 钙本身的水平受到控制。里奇韦医生和弗里曼医生 开发了一个强大的模型系统来解决这个问题 问题它利用了一种天然的发光蛋白质 在水螅虫胚胎中发现， 钙