Collaborative Research: Calcium in the Early Development of Hydrozoans

合作研究：水螅虫早期发育中的钙

• 批准号: 9207470
• 负责人: Ellis Ridgway
• 金额: $ 8万
• 依托单位: Virginia Commonwealth University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-01-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9207470&HistoricalAwards=false
• 关键词: Collaborative; Research; Calcium; Early; Development

The eggs and embryos of several species of hydrozoans contain endogenous calcium indicators similar to aequorin. These preparations offer unique opportunities for studying the role of calcium in early development. Two calcium transients, one at fertilization, and another at cleavage, are crucial for normal development. This project will employ photon counting techniques and image intensified video microscopy methods to determine if the fertilization calcium transient is due to a calcium wave, and microinjection experiments to determine what second messengers participate in its initiation and propagation. Cytokinesis in hydrozoans is slow and requires external calcium. Photon counting, fluorescent dye injection, and drugs will be used to investigate how the calcium transient during cytokinesis is regulated. Voltage dependent calcium channels first become functional about one hour after fertilization. These channels play a major role in rapid calcium influx but very little is known about the developmental events that lead to the onset of channel function. This project will define the factors that are responsible for initiating calcium channel function in hydrozoans. When calcium rises in the cytosol of cleavage stage embryos, calcium is simultaneously transported from the endoplasmic reticulum to the external medium. This project will identify the mechanism that mediates this calcium efflux.

几种水螅虫的卵和胚胎含有 内源性钙指标类似水母发光蛋白。这些 筹备工作提供了独特的机会， 钙在早期发育中的作用两个钙瞬变，一个在 受精，另一个在卵裂，是至关重要的正常 发展这个项目将采用光子计数技术 和图像增强视频显微镜方法，以确定 受精钙瞬变是由于钙波，并且 显微注射实验来确定第二信使 参与其发起和传播。胞质分裂 水螅虫是缓慢的并且需要外部钙。光子 计数，荧光染料注射，和药物将被用来 研究胞质分裂过程中钙瞬变是如何 监管.电压依赖性钙通道首先成为 受精后一小时左右开始发挥作用。这些通道 在快速钙内流中起主要作用， 了解导致发病的发育事件 通道功能该项目将定义 负责启动钙通道功能， 水螅虫。当卵裂期细胞质中钙离子升高时， 胚胎，钙是同时从运输 内质网到外部介质。该项目将 确定介导这种钙外流的机制。