Molecular Control of Ammonia Assimilation in Alfalfa Root Nodules

苜蓿根瘤氨同化的分子控制

• 批准号: 8905006
• 负责人: Carroll Vance
• 金额: $ 19.37万
• 依托单位: University of Minnesota Saint Paul
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-15 至 1993-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8905006&HistoricalAwards=false
• 关键词: Molecular; Control; Ammonia; Assimilation; Alfalfa

The objective of this research is to characterize the biochemistry and molecular biology of nitrogen assimilation of alfalfa (Medicago sativa) in symbiosis with the bacterium Rhizobium meliloti, and to determine the effect of the plant host in regulation of nodule formation and nitrogen fixation. The proposed research will test the hypothesis that assimilation of ammonia produced during nitrogen fixation involves the the expression of nodule.specific forms of enzymes of the nitrogen assimilation pathway, and will be pursued through analysis of the expression of genes for phosphoenolpyruvate carboxylase, glutamate synthase, and aspartate amino transferase in alfalfa strains that form nitrogen fixing and non.nitrogen fixing nodules. This research will increase knowledge of plant host contributions to nitrogen fixation. It has the potential to identify mechanisms of coordination of plant and bacterial gene expression involved in this agronomically and economically important process and, indirectly, to increase its efficiency.

这项研究的目的是表征苜蓿（Medicago sativa）在与细菌梅洛利蒂的共生中的氮同化（Medicago sativa）的生物化学和分子生物学，并确定植物宿主在结节形成和硝基原成的调节中植物宿主对植物宿主的作用。 提出的研究将检验以下假说：氮固定过程中产生的氨的同化涉及结节的表达。氮固定和非硝基固定结节。 这项研究将增加对植物宿主对氮固定的贡献的知识。 它具有鉴定植物和细菌基因表达的协调机制，涉及该农艺和经济上重要的过程，并间接地提高其效率。