Functional Analysis of the Broad- Complex in Drosophila

Guild博士正在研究一种基因表达程序， 对果蝇的发育很重要 黑腹菌本程序中基因的顺序表达 幼虫的唾液腺受类固醇激素的调节 蜕皮激素和收益通过级联的转录 调控宽复合体（BR-C）基因座编码多个 产品和行为早在这级联调节 下游（晚期）基因的表达。Guild医生会 描述BR-C产品，以了解这些早期 基因与晚期基因交流。通过遗传学方法 他应该能够识别哪些RNA产物在这个过程中起作用， 容量他将测试不同的BR-C产品发挥作用的想法 在不同但平行的组织特异性基因表达中的作用 通过开发针对不同BR-C的产品特异性探针， RNA和蛋白质。此外，他还将确定BR-C是否 产品以直接的方式与任何其他基因座相互作用， 唾液腺级联通过调查这个多线基因组， 结合BR-C产物。 %%% 这个建议是关于激素调节的表达 一个复杂的基因位点参与变态， 果蝇是研究发育的模式生物 遗传学

Dr. Guild is studying a program of gene expression which is important to the development of the fruit fly Drosophila melanogaster. Sequential expression of the genes in this program is the larval salivary gland is regulated by the steroid hormone ecdysone and proceeds through a cascade of transcriptional regulation. The Broad-Complex (BR-C) locus encodes multiple products and acts early in this cascade to regulate the expression of downstream (late) genes. Dr. Guild will characterize BR-C products in order to learn how these early genes communicate with late genes. By taking a genetic approach he should be able to identify which RNA products serve in this capacity. He will test the idea that different BR-C products play roles in different but parallel tissue specific gene expression programs by developing product specific probes for different BR-C RNAs and proteins. In addition, he will determine whether BR-C products interact in a direct way with any other loci in the salivary gland cascade by surveying this polytene genome for bound BR-C products. %%% This proposal is concerned with the hormone regulated expression of a complicated genetic locus involved in metamorphosis in Drosophila, a model organism for the study of developmental genetics.